Validation of the ID Screen PRRS Indirect ELISA

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and economically devastating porcine viral disease. The causative agent belongs to the genus Arterivirus, family Arteriviridea.

The ID Screen® PRRS Indirect ELISA is designed to detect antibodies directed against PRRSV in porcine serum and plasma.

This presents validation data for this test.

The ID Screen PRRS Indirect ELISA may be used with individual porcine serum or plasma (domestic swine or wild boar samples). The kit includes microplates coated with PPRSV recombinant proteins and an anti-porcine IgG horseradish peroxidase (HRP) conjugate.

1) Diagnostic specificity was evaluated on 950 samples from fattening pigs from Brittany, France. These herds had tested negative for PRRS over a number of years. 894 sera from breeders from France and other European countries were also tested. Specificity was measured to be 99.9 % (CI95%: 99.8 % - 100.0), n=1844.

2) 113 samples, randomly selected from infected herds in Europe, were tested in parallel with the ID Screen® ELISA and Kit A. 110 samples gave identical results on both tests. The measured percentage of correlation was 97.3%.

3) Eight 3-week old pigs were vaccinated intradermally with the PRRS Porcillis vaccine and challenged 31 days post vaccination with a PRRSV Brittany European strain. The 8 pigs were bled 7, 14 and 21 days post-vaccination (dpv). This study was performed by the French Reference Laboratory (ANSES, Ploufragan, France). The ID Screen® ELISA detected seroconversion between 14 and 21 dpv.

4) 535 sera from both disease-free and infected herds were tested in parallel using the ID Screen® ELISA and another widely used ELISA (Kit A). 530 samples gave identical results on both tests. Measured test agreement was found to be 99.1%.

5) The 2015 GD Deventer Proficiency Testing panel was tested. The ID Screen® ELISA correctly identified both European and American strains, and gave similar results to those obtained by other commercial PRRS ELISAs.

The ID Screen® PRRS Indirect ELISA kit demonstrates high specificity and excellent performance on reference panels. It efficiently detects positive animals in the field and correctly identified all strains.

The kit shows excellent agreement with other commercial ELISAs. With high test reproducibility and repeatability, the ID Screen® PRRS Indirect ELISA is a reliable tool for the detection of antibodies against PRRSV.

Disclosure of Interest: None Declared.