Effect of Isoquinoline Alkaloid on PRRS Immune Response in Weaning Pigs
Published:February 17, 2023
By:A. Boonsoongnern 1, S. Laopiem 1, P. Jirawattanapong 1, P. Udomprasert 1, T. Poolsawat 2, P. Poolperm 1,* / 1 Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom; 2 Phytobiotics (Thailand) Co.,Ltd., Bangkok, Thailand.
Porcine Reproductive and Respiratory Syndrome virus (PRRSv) has been reported to negatively modulate pig immune response, particularly the inhibition of interferon-g (IFN-g) and increasing the number of interleukin10 (IL-10) producing cells. Thus, one of the strategies in controlling the immune-modulation of PRRSv is to use immuno-enhancer for PRRS immune response. Sangrovit Extra ®, a plant extract from Macleaya cordata, mainly consists of isoquinoline alkaloids (IQs). The IQs induced biological activities; anti-inflammatory, anti-microbial and immunomodulatory activities. The purpose of this study was to investigate the effect of IQs on serum neutralizing (SN) antibody titer and specific IFN-g producing peripheral blood mononuclear cells (PBMC) to PRRSv in weaning pigs in Thailand.
Materials and Methods:
Twenty four, 21-day-old, male piglets, bought from a PRRS-free herd, were individually housed and divided into 4 groups, (n=6 per group); A (non-vaccinated, no challenged), B (non-vaccinated, with challenged), C (vaccinated, with challenged) and D (vaccinated, 100 ppm of IQs with challenged) groups. The C and D pigs were vaccinated with the PRRS vaccine (Ingelvac PRRS® MLV, BI, Germany) at 28 days of age. The B, C and D pigs were challenged with 2 ml of field PRRSv 105TCID50/ml and 107TCID50/ml intramuscularly on day 28 and 42 post vaccination, respectively. Blood samples were collected on day 0, 28, 35, 42 and 49 for serum neutralizing antibodies and PRRS specific IFN-g producing PBMC, measured by flow cytometry (BD AccuriTM C6).
Results:
The group D pigs (IQs 100 ppm) not only had the highest level of SN titer, in particular on D42 and D49, but also continuously increased percentage of specific IFN-g producing PBMC. Moreover, vaccinated pigs were found to have a higher level of both SN titer and the percentage of IFN-g producing PBMC than unvaccinated pigs. After the PRRSv challenge, percentage of specific IFN-g producing PBMC had increased drastically in vaccinated piglets compared to the slower response of SN titer.
Conclusion:
Pigs fed Sangrovit Extra® supplemented feed enhanced both SN titer and specific IFN-g producing PBMC in PRRS vaccinated piglets compared to non-vaccinated pigs. In conclusion, the protective immune response to the PRRS infection should be focused more on IFN-g producing cells than the SN titer, particularly in vaccinated piglets.
Disclosure of Interest: None Declared.
Published in the proceedings of the International Pig Veterinary Society Congress – IPVS2016. For information on the event, past and future editions, check out https://ipvs2024.com/.