Nucleotides from yeast extract: potential to replace animal protein sources in food animal diets

Published on: 03/06/2015
Author/s : G. WALTER TIBBETTS - Alltech Inc.

For years animal proteins have been used in livestock diets without many questions; however fishmeal, meat and bone meal, blood meal, blood plasma and even milk by-products in some cases are now coming under public scrutiny. This is in part due to a lack of public confidence stemming from appearance of BSE and other disease scares along with salmonella and E. coli contamination of animal food prod...

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April 17, 2018

I would like to ask about the best way to get the pure nucleotides from yeast? I mean the best manufacture protocol?

June 29, 2021
Generally speaking, there are three methods: autolysis, enzymatic hydrolysis, acid hydrolysis

The autolysis method is used the yeast cell's own enzyme to dissolve the cell wall and release the nutrients in the yeast; while the enzymatic method achieves this effect by adding external enzymes.

The advantage of the autolysis method is low cost and can get a good flavor.

The advantage of enzymatic hydrolysis is good quality but it's at a high cost. This method is generally used to produce high-end YE products.

The advantages of acid hydrolysis are high extract yield and large amounts of free amino acids. However, it has the disadvantage of poor palatability, high salt content, high post-processing cost, and high pollution. So it's rarely used.

Nowadays the most often used method is a combination of autolysis-enzymatic hydrolysis process, it's adopted by many manufacturers in China and U.S.A.

For example, Hiyeast from China is one of those manufacturers.

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Scott Webster
Scott Webster
Pork Industry Expert Advisor
  Madison, Wisconsin, United States
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