Interaction between vitamin B6 and source of selenium on the response of the selenium-dependent glutathione peroxidase system to oxidative stress induced by oestrus in pubertal pig

Published on: 2/10/2020
Author/s : Danyel Bueno Dalto 1,2; Mélanie Roya, 3; Isabelle Audet 1; Marie-France Palin 1; Frédéric Guay 3; Jérôme Lapointe 1; J. Jacques Matte 1. / 1 Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada; 2 Department of Animal Science, Universidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil; 3 Department of Animal Science, Laval University, Quebec, Quebec G1V 0A6, Canada.
Summary

Abstract

This study aimed to assess the interaction between vitamin B6 and selenium (Se) for the flow of Se towards the Se-dependent glutathione peroxidase (GPX) system in response to oxidative stress naturally induced by oestrus in a pubertal pig model. At first oestrus, forty-five gilts were randomly assigned to the experimental diets (n = 9/group): basal diet (CONT); CONT + 0.3 mg/kg of Na-selenite (MSeB60); MSeB60 + 10 mg/kg of HCl-B6 (MSeB610); CONT + 0.3 mg/kg of Se-enriched yeast (OSeB60); and OSeB60 + 10 mg/kg of HCl-B6 (OSeB610). Blood samples were collected at each oestrus (long-term profiles), and daily from day −4 to +3 (slaughter) of the fourth oestrus (peri-oestrus profiles) after which liver, kidneys, and ovaries were collected. For long-term profiles, CONT had lower blood Se than Se-supplemented gilts (p < 0.01) and OSe was higher than MSe (p < 0.01). Lower erythrocyte pyridoxal-5- phosphate was found in B60 than B610 (p < 0.01). No treatment effect was observed on GPX activity. For peri-oestrus profiles, treatment effects were similar to long-term profiles. Treatment effects on liver Se were similar to those for long-term blood Se profiles and OSe had higher renal Se concentrations than MSe gilts (p < 0.01). Gene expressions of GPX1, GPX3, GPX4, and selenocysteine lyase in liver and kidney were greatest in OSeB610 gilts (p < 0.05). These results suggest that dietary B6 modulate the metabolic pathway of OSe towards the GPX system during the peri-oestrus period in pubertal pigs.
 
Key words: Gilt, Glutathione peroxidase, Oestrus Pyridoxine, Inorganic selenium, Organic selenium.
 

Abstract published in Journal of Trace Elements in Medicine and Biology 32 (2015) 21–29. http://dx.doi.org/10.1016/j.jtemb.2015.05.002.
 
Author/s
Veterinary, specialist in agribusiness, with MSc. and PhD. in Animal Science (nutritional additives for weaned pigs and mineral/vitamin nutrition for gestating sows, respectively). Currently, He´s a post-doctorate fellow at Agriculture and Agri-Food Canada working with minerals and vitamins for neonate pigs and also, the bioavalability of vitamin B12 in growing pigs as a model for human nutrition.
 
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