Josiah Liew

I agree, it is a very important topic. On point was not discussed enough I think: standardisation of the bioinformatics. While new developments are always happening overall, the 16s sequencing bioinformatics is now mostly established to use ASV or mOTUs based on the DADA2 pipeline. It might be useful to agree at least on a best practice and recommended tools for the bioinformatics as well. This wi ...

This is an important issue. It will be interesting to see/hear how similar the results are from different laboratories using the proposed protocol when analysing the same mock communities or sample(s) in developing/validating the standardised methodology.