Zoila Coloma

ELISA is not validated for finished feed, we do not even recommend ELISA for testing of finished feed. Never less, it is the best method for testing raw materials. You can always validate your ELISA equipment and method against reference methods as LC MS/MS or HPLC.

Depends on the kit assay. Sometimes screening kits may provide false positive reactions. But you can use positive and negative controls along with calibration standards and QC for more accurate results. You can also compare with cross reference method of LC-MS/MS which is more expensive. Hope this will be helpful.