Brucellosis and Bovine Tuberculosis (TB) are a constraint to livestock production in Quetta. Bovine tuberculosis (TB) is a contagious neglected zoonosis of cattle that is prevalent but under-investigated in Quetta. Brucellosis is an infectious disease affecting mainly sexually mature animals and is caused by organisms of the genus Brucella. Brucella abortus is the specie that mainly affects cattle throughout the world, while Brucella melitensis affects mainly goats and sheep. The aim of this research was to determine the prevalence of brucellosis and Bovine tuberculosis in cattle, goats and sheep. The total size of sample was 500 animals. Amongst which 185(37%) were sheep, 180(36%) were goat, 109 (21.8%) were cow, 18(3.6%) were buffalo, 4(1.2%) were bull and 2 (1.2%) were calf. All the samples from the study area were tested for Brucella and Bovine tuberculosis. The apparent prevalence for Brucella found were as follows: 3(n=109) in cow, 2(n=185) in sheep, 2(n=18) in buffalo and 1 (n=180) in goat. There were no positive tests for Bovine tuberculosis. A more complete understanding of the costs of the disease and the costs and benefits of control measures would promote broader application of the most efficient and effective control measures, contributing to improved animal and human health, better livelihood outcomes for the poor and macroeconomic growth.