Explore

Communities in English

Advertise on Engormix

HISTOPATHOLOGICAL AND SEROLOGICAL STUDIES ON PARATUBERCULOSIS IN CATTLE AND BUFFALOES

Published: November 19, 2011
By: A. Sikandar, AH Cheema, M Younus, A Aslam, MA Zaman, T Rehman
Summary
Paratuberculosis (Johne’s diseases) is responsible for massive economic losses to dairy industry, both in the industrially advanced as well as in the eveloping countries. To detect its occurrence in cattle and buffaloes locally, Blood and tissue samples from clinically weak and grossly suspected slaughtered animals were collected from two abattoirs of Jhang, Municipal Area, Pakistan. Acid-fast smear staining, Gross/Histopathology and indirect ELISA was done for the detection of Mycobacterium avium subsp. paratuberculosis (MAP). Total 134 samples illustrating gross pathological lesions were collected, only 11.19% (Cattle:6.67%, Buffaloes:12.5%) showed acid fast bacilli through smear staining and were taken as confirmed cases. Thickening of intestines alone was not a reliable indicator of Johne’s disease. Tissue sections from intestines and mesenteric lymph nodes from these Acid fast positive animals were stained with Hematoxylin & Eosin (H&E) and Ziehl Neelsen (ZN) methods. Sum of (15/134) impression smear staining as well as (15/15) tissue sections of the intestines were found ZN positive, and only 6.7% (09/134) of impression smears and 100% (09/09) of tissue sections of mesenteric lymph nodes showed acid fast bacilli. Through ELISA, Two cattle and five buffaloes (07/134) gave positive optical densities, while one cattle and seven buffaloes (08/134) were judged as doubtful. It is concluded that infection of MAP can be identified by Histopathology and ELISA. The present study was the first record of paratuberculosis among the dairy animals slaughtered at Jhang abattoirs. Keywords: Cattle; Buffalo; Paratuberculosis; Mycobacterium avium subsp. paratuberculosis; Histopathology; ELISA.
Content from the event:
Authors:
Arbab Sikandar
Recommend
Comment
Share
Profile picture
Would you like to discuss another topic? Create a new post to engage with experts in the community.
Featured users in Dairy Cattle
Jim Quigley
Jim Quigley
Cargill
Technical Lead - Calf & Heifer at Cargill
United States
Pietro Celi
Pietro Celi
DSM-Firmenich
DSM-Firmenich
United States
Mauricio Grierson
Mauricio Grierson
MSD - Merck Animal Health
MSD - Merck Animal Health
United States
Join Engormix and be part of the largest agribusiness social network in the world.