EFFECT OF AMOXICILLIN TRIHYDRATE ON THE PHARMACOKINETICS OF KETOPROFEN WHEN CO-ADMINISTERED PARENTERALLY IN HEALTHY PAKISTANI BUFFALOES

Published: November 19, 2011

By: Z. U. Rehman, M. Ashraf, M. A. Khan, M. A. Jabbar and M. A. Rasheed

Summary

The purpose of present investigation is to study the effect of Amoxicillin Trihydrate on the pharmacokinetic parameters of Ketoprofen, when both the drugs are co-administered parenterally in healthy Pakistani buffaloes. For this purpose, eight healthy buffaloes were taken and administered an intravenous injection of ketoprofen at the dosage of 3.0 mg/kg body weight through jugular vein. Blood samples (3-5ml) of buffaloes were drawn in vacutainers, pre-medication at zero-hr, 0.08, 0.17, 0.25, 0.5, 0.75, 1.0, 2.0, 3.0, 4.0, 6.0, 8.0, 10, 12.0, 24, 48, 60, 72, 84 and 96 hrs post medication. After a wash out period of 14 days, the same buffaloes were administered first an intramuscular injection of amoxicillin trihydrate at the dosage of 15 mg/kg body weight and after the lapse of 10 minutes, an intravenous injection of ketoprofen at the dose of 3.0 mg/kg body weight through jugular vein. Blood samples (3-5ml) of buffaloes were drawn according to the schedule described above. The concentration of ketoprofen in plasma was measured by a validated HPLC (high performance liquid hromatography) method. By taking the ketoprofen plasma concentration versus time data, the pharmacokinetic parameters of ketoprofen were calculated as Mean ±SEM AUC (Area Under the concentration time Curve) ± μg.h.ml-1, Cl (Clearance) ± L.hr-1.Kg-1, t½ (Half Life) ± hr-1, VD (Volume of Distribution) ± L-1, VDss (Volume of distribution at Steady State) ± L.Kg-1, and Kel (Elimination Rate Constant) ± L.hr-1 respectively. The results of this study indicated that Amoxicillin Trihydrate has no significant change the pharmacokinetics parameters of Ketoprofen when coadministered parenterally. Keywords: Amoxicillin Trihydrate, Ketoprofen, Pharmacokinetics, Buffaloes, Pakistan

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