A research study was designed to investigate effect of different feed combinations on milk composition and blood metabolites with feed intake based on different kharif crops and feed concentrates fed to dairy cows in Peshawar region. The animals were divided into 5 groups and five different feed combinations were fed to them. Milk and blood samples were analyzed using commercial kits. The effect of feed on blood metabolites and milk composition was highly significant (P = 0.000). Highest blood glucose values were observed in group B followed by group E, A, D and C. Similarly, highest blood protein values were observed in group D, followed by group C, B, E & A with mean values of 93.32,93.23,90.69, 89.30 and 87.60 respectively. Blood Triglycerides were higher in group D followed by group C , E and A with mean values of 11.06,10 .29 ,10. 03 ,9 .77 & 9.77 respectively. Highest milk fat values were were recorded in animals of group D followed by C, B, A and E with mean values of 6.90,6.84,6.37,6.35 and 6.27 respectively. Animals of group D were noted for their highest milk Lactose values followed by C, B ,A and E with mean values of 3.74,3.71,3.3 45 and 3.4 and 3. 38 respectively. Similarly, highest milk protein values were attained by animals of groups D followed C , A and E with mean values of 2.65,2.62,2.45,2.41 and 2.41 respectively. Different feed combinations had ignificant correlation with blood Triglycerides. Blood Glucose was correlated with blood protein, triglycerides, milk lactose, protein, fat, milk yield and SNF. Blood Triglycerides was significantly correlated with milk lactose, protein, fat, milk yield and SNF. It is suggested that different feed combinations influence the milk yield and composition and blood metabolites probably through influencing biological reactions in the body. Highest blood glucose were maintained by dairy cows more than 75% exotic blood levels and highest milk lactose levels were exhibited by buffaloes. Blood glucose and milk lactose levels showed an opposite pattern in NPNS (non-pregnant non-served), NP (nonpregnant) and pregnant dairy animals (R2-0.81, 0.99). Keywords: Milk Composition, Blood Metabolites, Feed combinations