The aim of this experiment was to investigate the effect of aflatoxin B (AFB) in the diets of Nile tilapia (Oreochromis niloticus) and to examine the detoxification activity of a commercial anti-mycotoxin ARCAVIT Bioacid Forte. The experimental period lasted 70 days (10 weeks) during September - December 2018. Sixty fish were chosen with an average initial weight of about 40 g then distributed randomly into three treatments in six glass aquaria (30 x 60 x 40cm), each treatment was applied in two aquaria (replicates). Aflatoxin B (AFB) was added at a concentration of 5 ppb. The anti-mycotoxin ARCAVIT ((pro- and prebiotics) is one of the commercial anti-mycotoxin in the local market; it is the first test for it in aquaculture) was added at a concentration of 0.5 g/Kg. The experimental diets of the three treatments were The control treatment (T1): the basal diet without any additives. The second treatment (T2): the basal diet with addition of 5 ppb AFB. The third treatment (T3): the basal diet with addition of 5 ppb AFB and 0.5g/Kg ARCAVIT. All the experimental groups were fed the experimental diets at a daily rate of 3% of the live body weight of the fish, at two meals. The obtained results could be summarized in the following points:
Conclusion: Aflatoxin B is very toxic, even at a low concentration as 5 ppb (ng/g), for Nile tilapia. It threat fish morphology, gross pathology, liver histology, growth performance and internal organs indices, feed utilization, body composition and properties, as well as blood chemistry. Moreover, the anti-toxic agent at the tested level did not completely overcome the aflatoxicity symptoms. That leads to keep the old wisdom "PROPHYLAXIS IS MORE BETTER THAN CURING".
Abdallah, M.F.; G. Girgin and T. Baydar (2015). Occurrence, Prevention and Limitation of Mycotoxins in Feeds. Animal Nutrition and Feed Technology, 15: 471-490.
Abdallah, M.F.; G. Girgin, T. Baydar, R. Krskaa and M. Sulyoka (2017). Occurrence of multiplemycotoxins and other fungal metabolites in animal feed and maize samples from Egypt using LC-MS/MS. Published online in Wiley Online Library: (wileyonlinelibrary.com) DOI 10.1002/jsfa.8293.
Abdel Rahman, A. N., Abdellatief, S. A., and Mahboub, H. H. H. (2017). Protection of Nile tilapia, Oreochromis niloticus from aflatoxin B1 toxicity by dietary supplementation with Fennel essential oil and Saccharomyces cerevisiae. The Egyptian Journal of Aquatic Research, 43 (3): 235-240.
Abdelhamid, A. M. (1983). Mykotoxin-Nachweis in Lebens-und Futtermitteln des subtropischen Klimas. In: Kurzfassungen der Vorträge zur 37. Tag. Ges. Ernährungsphysiol. Haustiere, Gottingen, 5. Z. Tierphysiol., Tierernährug u Futtermittelkde., 50: 4-5 (Egyptian Agricultural Bibliography, IX, 1986, 85-100438).
Abdelhamid, A.M. (1996). Field and Laboratorial Analysis in Animal Production. Dar Al-Nashr for the Egyptian Universities. Cairo, 680p., 977-5526-47-7, Deposition No. 11318/1996.
Abdelhamid, A. M., (2000). Pollution of aquatic environment. Proc. Symposium “Fish Wealth Development-Principles and Limitations”, Mansoura Univ., May 9, PP: 225-233.
Abdelhamid, A. M. (2004). Aquafeed Quality: Mycotoxins and Mycotoxicoses in Fish. Nutrition in the Management of Aquaculture System, 14-24 June, World Fish Center/ FAO. World Fish Center, Abbassa- Abou Hammad- Sharkia- Egypt.
Abdelhamid, A. M. (2009). Thirty years (1978-2008) of mycotoxins research at Faculty of Agriculture, Mansoura University, Egypt. Egyptian Journal of Nutrition and Feeds, 12 (1): 1-14.
Abdelhamid, A. M. (2010). Thirty-two years (1978 – 2010) of mycotoxins research at Faculty of Agriculture, Mansoura University, Egypt. Abstracts book of MycoRed Workshop on "Mycotoxicological risks in Mediterranean countries: economic impact, prevention, management and control", 25-27 Oct., Cairo, Egypt.
Abdelhamid, A.M. (2019a). Husbandry, Breeding, Physiology and Diseases of Fish. 625p, Deposition No. 25440/2018.
Abdelhamid, A.M. (2019b). Fish Nutrition. 469p, Deposition No. 25442/2018.
Abdelhamid, A. M. and K. I. Mahmoud (1996). Elimination or adsorption of aflatoxin from poultry feedstuffs. Proc. Food Borne Contamination and Egyptian’s Health Conference, Mansoura Univ., 26-27 Nov., PP: 61-69.
Abdelhamid, A. M., A. E. Abdel- Khalek, A.I. Mehrm and F. F. Khalil (2004a). An attempt to alleviate aflatoxicosis on Nile tilapia fish by dietary supplementation with chicken-hatchery by-products (egg shells) and shrimp processing wastes (shrimp shells) on: 1- Fish performance and feed and nutrients utilization. J. Agric. Sci. Mansoura Univ., 29: 6157 – 6173.
Abdelhamid, A. M., A. E. Abdel- Khalek, A. I. Mehrm and F.F.Khalil (2004b). An attempt to alleviate aflatoxicosis on Nile tilapia fish by dietary supplementation with chicken-hatchery by-products (eggshells) and shrimp Processing wastes (shrimp shells) on: 2 - Clinical, blood and histological parameters. J. Agric. Sci. Mansoura Univ., 29: 6175 – 6196.
Abdelhamid, A. M., A. I. Mehrim and F. F. Khalil (2004c). Detoxification of aflatoxin-contaminated diet of tilapia fish using dietary supplementation with eggshell, Betafin®, clay or silica. J. Agric. Sci. Mansoura Univ., 29: 3163 – 3174.
Abdelhamid, M. A.; A. M. Ahmed and Kh. M. El-Meleigy (2002a). Detoxification of aflatoxins - contaminated diet by some physical and chemical means. J. Agric. Sci. Mansoura Univ., 27: 8213 - 8224.
Abdelhamid, A. M., E. A. Sadik and E. A. Fayzalla (1985). Preserving power of some additives against fungal invasion and mycotoxin Production in stored-crushed-corn containing different levels of moisture. Acta Phytopathologica Academca Scientiarum Hungaricae, 20: 309-320.
Abdelhamid, A. M., F. F. M. Khalil and M.A.A. Seden (2000). Possibility of using dried live yeast and lacto sacc in Nile tilapia fingerlings' diets. J. Agric. Sci. Mansoura Univ., 25 (8): 4905-4911.
Abdelhamid, A. M., F. F. M. Khalil, M. I. El-Barbary, V. H. Zaki, and H. S. Husien (2002b). Feeding Nile tilapia on Biogen® to detoxify aflatoxic diets. Proc. 1st Ann. Sc. Conf. Anim. & Fish Prod., Mansoura Fac. Agric., 24 & 25 Sep., PP: 207-230.
Abdelhamid, A. M., F. I. Magouz, M. F. E. Salem, A. A. Mohamed, and M. K. Mohsen (2002c). Effect of dietary graded levels of aflatoxin B1 on growth performance and biochemical, chromosomal and histological behavior of Nile tilapia, Oreochromis niloticus. Proc. 1st Ann. Sc. Conf. Anim. & Fish Prod., Mansoura Fac. Agric., 24 & 25 Sep., PP: 231-250.
Abdelhamid, A. M., M. F. I. Salem, A. I. Mehrim, and M. A. M. El-Sharawy (2007). Nutritious attempts to detoxify aflatoxic diets of tilapia fish: 1-Fish performance, feed and nutrients utilization, organs indices, residues and blood parameters. Egypt. J. Nutr. Feeds, 10, 205-223.
Abdelhamid, A. M., S. A. El-Ayoty and H. H. Elsaadany (1992). The influence of contamination with separate mycotoxins (aflatoxins, ochratoxin A, citrinin, patulin, penicillic acid or sterigmatocystin) on the dry matter and organic matter digestibility of some roughage (berseem hay and wheat straw) using in vitro rumen fermentation. Arch. Anim. Nutr., 42:179-185.
Abdelhamid, A. M., Y. M. Shabana and S. S. A. Gomaa (2006). Aquatic fungi and fish production in Egypt. The 2nd Inter. Sci. Con. For Environment "Recent Environmental Problems and Social Sharement", 28-30 March, South Valley University. PP: 488 – 523.
Abdelhamid, A. M., Y. M. Shabana and S. S. A. Gomaa (2008). Aquatic fungi and fish production in Egypt, I- in vitro studies. J. Agric. Sci. Mansoura Univ., 33: 4887 – 4899.
Abdelhamid, A. M.; Y. M. Shabana and Sahar S. A. Gomaa (2009). Aaquatic fungi and fish Production in Egypt, II- in vivo studies. J. Agric. Sci. Mansoura Univ., 34: 7675 – 7686.
Ahamad, d.B.; A.K. Sharma and P. Dwivedi (2015). Aflatoxin B1 induced carcinogenicity in Wistar rats: Clinical signs and growth performance. Shanlax International Journal of Veterinary Science, 3 (2): 2321-6387.
Aiko, V. and A. Mehta (2015). Occurrence, detection and detoxification of mycotoxins. J. Biosci., 40: 943–954.
Ali, B.A. (2008). Effect of total replacement of fish meal by plant protein source and amecozyme on growth performance and feed utilization of monosex Nile tilapia (Oreochromis niloticus) fingerlings. Egypt. J. of Appl. Sci., 23 (7): 13-24.
Aly, S.M.; F.M. Mohamed and G. John (2008). Effect of probiotics on the survival, growth and challenge infection in tilapia nilotica (0reochromis niloticus). Aquaculture Research, 39: 647-656.
Anater, A.; L. Manyes, G. Meca, E. Ferrer, F.B. Luciano, C.T. Pimpão and G. Font (2016). Mycotoxins and their consequences in aquaculture: A review Aquaculture, 451: 1–10.
Anikuttan, K. K., K. C. George, N. K. Sanil and K. S. Sobhana (2018). Hepatic lesions associated with induced aflatoxicosis in the estuarine teleost Etroplus suratensis (Bloch, 1790). Indian J. Fish., 65 (3): 57-65.
AOAC. (1990). Official Methods of Analysis Chemists. AOAC International, Washington DC, USA.
Ayyat, M. S., A. M. N. Ayyat, A. A. Al-Sagheerand and A. E. A. M. El-Hais (2018). Effect of some safe feed additives on growth Performance, blood biochemistry, and bioaccumulation of aflatoxin residues of Nile tilapia fed aflatoxin-B1 contaminated diet. Aquaculture, 495: 27-34.
Clement, S., and R. T. Lovell (1994). Comparison of processing yield and nutrient composition of cultured Nile tilapia (Oreochromis niloticus) and channel catfish (Ictalurus punctatus). Aquaculture, 119 (2-3): 299-310.
Decie, S. I. V. and S. M. Lewis (2006). Practical Hematology. 10th Edn., Churchill Livingstone, London. ISBN: 13: 978- 443, PP: 736.
Deng, S. X., , L. X. Tian, F. J., Liu, S. J., Jin, G. Y., Liang, H. J. Yang and Y. J. Liu (2010). Toxic effects and residue of aflatoxin B1 in tilapia (Oreochromis niloticus×O. aureus) during long-term dietary exposure. Aquaculture, 307 (3-4): 233-240.
Duncan, D.B. (1955). Multiple ranges and multiple F-tests. Biometrics, 11: 1-42.
El-Barbary, M.I. (2018). Impact of garlic and curcumin on the hepatic histology and cytochrome P450 gene expression of aflatoxicosis Oreochromis niloticus using RT-PCR. Turkish Journal of Fisheries and Aquatic Sciences, 18: 405-415.
El-Barbary, M. I. and A.I. Mehrim (2009). Protective Effect of Antioxidant Medicinal Herbs, Rosemary and Parsley, on Subacute Aflatoxicosis in Oreochromis niloticus. Journal of Fisheries and Aquatic Science, (4): 178-190.
El-Barbary, M.I. and M.H. Mohamed (2014). Chemoprevention and therapeutic efficacy of glutathione against aflatoxicosis in Nile tilapia (Oreochromis niloticus). Global Vetrinaria, 13: 1111-1121.
El-Boshy, M. E., A. M. M., El-Ashram and N. A. A. El-Ghany (2008). Effect of dietary beta-1, 3 glucan on immunomodulation on diseased Oreochromis niloticus experimentally infected with aflatoxin B1. In 8th international symposium on Tilapia in aquaculture (PP. 1109-1127).
El-Dakar, A.Y. (2004). Growth response of hybrid tilapia, Oreochromis niloticus x Oreochromis auraus, fingerlings to diets supplemented with different levels of caraway seeds. J. Agric. Sci. Mansoura Univ., 29 (11): 6083-6094.
El-Ebiary, E.H. and M.A. Zaki (2003). Effect of supplementing active yeast to the diets on growth performance and blood constituents of mono-sex tilapia (Oreochromis niloticus). Egypt. J. Aquat. Biol. & Fish., 7 (1): 127-139.
Eweedah, N.M.; E.M. Abd El-Raouf, M.F.I. Salem, M.M.E. Khalafalla and B.S. Abd El-Aty (2006). Replacement of fish meal by fresh water crayfish meal (Procombrus clarkii) in practical diets for Nile tilapia (Oreochromis niloticus). Egypt. J. Agric. Res., 84 (1B): 325-338.
Farrag, F.H.; F.F. Khalil, A.I. Mehrim and M.M.A. Refaey (2013). Pawpaw (Carica papaya) seeds powder in Nile tilapia (Oreochromis niloticus) diet. 1- Growth performance, survival, feed utilization, carcass composition of fry and fingerlings. J. Animal and Poultry Prod., Mansoura Univ., 4 (6): 363-379.
Henry, R.J. (1964). Colorimetric determination of total protein. Clinical Chemistry. Harper and Row Publ., New York, USA, P 18.
Hussain, D., A. Mateen and D. M. Gatlin III (2017). Alleviation of aflatoxin B1 (AFB1) toxicity by calcium bentonite clay: Effects on growth performance, condition indices and bioaccumulation of AFB1 residues in Nile tilapia (Oreochromis niloticus). Aquaculture, 475: 8-15.
Hussein S.Y. and M.A. Kobeisy (2001). Influence of dietary zinc and vitamin A levels on growth performance, blood constituents and immune competenceof Nile tilapia, Oreohromis niloticus under Upper Egypt conditions. Assiut Vet. Med. J., 45 (90): 55-74.
Isaka, M., M. Sappan, J. Jennifer Luangsaard, N. L., Hywel-Jones, S. Mongkolsamrit and S. Chunhametha (2011). Chemical taxonomy of Torrubiella s. lat.: zeorin as a marker of Conoideocrella. Fungal Biology, 115 (4-5), 401–405. doi:10.1016/j.funbio.2011.02.007.
Khattab, Y.A.E; A.M.E. Shalaby, S.M. Sharaf, H.I. El-Marakby and E.H. RizkAlla (2004). The physiological changes and growth performance of the Nile tilapia Oreochromis niloticus after feeding with Biogen® as growth promoter. Egypt. J. Aquat. Biol. & Fish., 8 (2): 145-158.
Kovac, T.; I. Borišev, B. Crevar, F. C. Kenjeric1, M. Kovac, I. Strelec, C. N. Ezekiel, M. Sulyok, R. Krska and B. Šarkanj (2018). Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus. Scientific Report, 8:12855 | DOI:10.1038/s41598-018-31305-9, 8p.
Kumar, S.; A. Sinha, S. Meshram, M. K. Singh, V.Singh and K.S. Hooda (2018). Myco-toxins Monitoring Device and their Management Strategies through Detoxifying Agents in Feed. Int. J. Curr. Microbiol. App. Sci., 7 (1): 3410-3426.
Magouz F. I., Eweedah, N. M., Salem, M. F. E. and A. A. Amer (2016). Detoxification of aflatoxin contaminated ration by chemical, biological and spices methods in Nile tilapia (Oreochromis niloticus) diets. J. Agric. Res. Kafr El-Sheikh Univ. PP: 102-119, Vol. 42(4)
Mahfouz, M. E. and A. H. Sherif (2015). A multiparameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status. The Journal of Basic and Applied Zoology, 71: 48-59.
Mass, J. (2018). Effect of mycotoxins on gut development. Gut Health, December 2018: 71-72.
McGowan, M.W.; J.D. Artiss, D.R. Standbergh and B.A. Zak (1983). Peroxidasecoupled method for colorimetric determination of serum triglycerides. Clin. Chem., 29: 538.
Mehrim, A.I. and M.F. Salem (2013). Medicinal herbs against aflatoxicosis by Nile tilapia (Oreochromis niloticus): Clinical, postmortem signs and liver histological patterns. Egypt. J. Aquac., 3 (1): 13-25.
Mehrim, A.I., M.M. Refaey, and Kh. M. Elmeleigy (2016). Glutathione-enhancer™ against foodborne aflatoxicosis of Oreochromis niloticus (Linnaeus, 1758). Journal of Fisheries and Aquatic Science, 11 (2): 131 – 146.
Mehrim, A.I.; A.M. Abdelhamid, A. Abou-Shousha, M.F.I. Salem and M.A.M.M. El-Sharawy (2006). Nutritious attempts to detoxify aflatoxic diets of tilapia fish: 2- Clinical, biochemical and histological parameters. Journal of the Arabian Aquaculture Society, 1 (2): 69-90.
Natt, M.P. and C.A. Herrick (1952). A new blood diluent for counting erythrocytes and leucocytes of the chicken. Poultry Science, 31: 735–738.
Neeratanaphan, L. and B. Tengjaroenkul (2018). Protective effects of Thai bentonite on aflatoxin B1 contaminated in diet of tilapia fish. Livestock Research for Rural Development, 30 (8) 14p.
Newberne, P.M.; W.W. Carlton and G.N. Wogan (1964). Hepatomas in rats and hepatorenal injury in ducklings fed peanut meal or Aspergillus flavus extract. Path. Vet., 1: 105-132 (Downloaded from vet.sagepub.com by guest on October 11, 2013).
NRC (National Research Council) (1993). Nutrient Requirements. National Academy Press. Washington. D. C., USA.
Press, C.McL. and Ø. Evensen (1999). The morphology of the immune system in teleost fishes. Fish & Shellfish immunology, 9: 309 – 318.
Saad, A.S. (2010). Effect of fermented diets on growth, body composition and survival of Nile tilapia "Oreochromis niloticus". J. Egypt. Ger. Soc. Zool., 61A: Comparative Physiology, 49-62.
Saei, M.M.; H. M. Taee, S. Siahpoust and M. Taheri (2017). Effects of Toxin Binder Biotox on Growth Performance Survival, Enzymatic Activity, Hematologichal and Biochemical Parameters of Fingerlings Rainbow Trout (Oncorhynchus mykiss) Fed Diets- Contaminated with Aflatoxin. J. Aquac. Res. Development, S2: 013. doi:10.4172/2155-9546.S2-013.
Salem, M.F.I.; M.M.E. Khalafalla, I.A.I. Saad and A.M.A. El-Hais (2008). Replacement of fish meal by silkworm, Bombyx mori pupae meal, in Nile tilapia, Oreochromis niloticus diets. Egtptian J. Nutrition and Feeds, 11 (3): 611-624.
Shahafve, S., M. Banaee, B. N. Haghi and M. Mohiseni (2017). Histopathological study of common carp (Cyprinus carpio) fed aflatoxin-contaminated diets. Int. J. Aquat. Biol., 5(2): 63-70.
Shalaby, A. M. (2009). The opposing effect of ascorbic acid (vitamin C) on ochratoxin toxicity in Nile tilapia (Oreochromis niloticus). Acta Polenica, 2: 18-22.
Shehata, T.M. and A.M. Goda (2000). Effect of dietary lipids levels and sources on immune response of Nile tilapia Oreochromis niloticus broodstock in winter season. Egypt. J. Aquat. & Fish., 4 (2): 1-25.Soltan et al., 2002
Soltan, M.A.; M.A. Hanafy and M.I.A. Wafa (2008). An evaluation of fermented silage made from fish by-products as a feed ingredient for African catfish (Clarias gariepinus). Global Veterinaria, 2 (2): 80-86.
Soltan, M.A.; K.A. Mohamed and A.H. Eid (2006). Effect of protein to energy ratio on growth performance and body composition of red tilapia reared in freshwater. Journal of the Egyptian Aquaculture Society, 1: 57-68.
SPSS (2017). Statistical Package for Social Science (for Windows). Release 25
Copyright ©, SPSS Inc., Chicago, USA.
Thophon, S., M. Kruatrachue, E.S. Upatham, P. Pokethitiyook, S. Sahaphong and S. Jaritkhuan (2003). Histopathological alterations of white sea bass, Lates calcarifer in acute and subchronic cadmium exposure. Environ. Pollut., 121: 307–320.
Tietz, N.W. (1986). Textbook of clinical chemistry. W.B. Saunders, Philadelphia, 1271.
Tietz, N.W. (1990). Clinical Guide to Laboratory Tests 2nd ed. Philadelphia.
Toguyeni, A., B. Fauconneau, T. Boujard, A. Fostier, E.R. Kuhn, K.A. Mol and J.F. Baroiller (1997). Feeding behaviour and food utilisation in tilapia, Oreochromis niloticus: effect of sex ratio and relationship with the endocrine status. Physiology and behavior, 62 (2): 273-279.
Wotton, I.D. and H. Freeman (1982). Microanalysis in Medical Biochemistry. Churchill, New York, USA.
Zaki, M. S. and O. Fawzy (2012). Effect of Afla-Toxins B1 on Endocrine Status in Cat fish (Clarious lazera). Life science journal-acta zhengzhou university overseas edition, 9 (1): 419-422.
Zychowski, K.E., A.R. Hoffmann, H.J. Ly, C. Pohlenz, A. Buentello, A. Romoser, D.M. Gatlin and T.D. Phillips (2013). The effect of aflatoxin-B1 on Red Drum (Sciaenops ocellatus) and assessment of dietary supplementation of NovaSil for the prevention of aflatoxicosis. Toxins, 5: 1555–1573.
Zychowski, K.E.; C. Pohlenz, T. Mays, A.H. Romoser, B.A. Michael, D.M. Gatlin III and T.D. Phillips (2012). The effect of NovaSil dietary supplementation on the growth and health performance of Nile tilapia (Oreochromis niloticus) fed aflatoxin-B1 contaminated feed. Aquaculture, doi: 10.1016/j.aquaculture.2012.11.020.