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Immunoaffinity Column Cleanup

Published: August 23, 2011
Source : Diego Montemayor, DVM (VICAM)
The Business Value Officially Validated Mycotoxin Test Methods
As the cleanup approach of choice in an expanding range of officially validated mycotoxin test methods, immunoaffinity columns offer food and feed industry stakeholders a proven approach to minimizing the costly risks of analytic errors. Available in user-friendly fluorometric formats, VICAM's commercial immunoaffinity test kits are designed to provide businesses and small testing labs with analytic data that meet the exacting standards of accuracy, precision, and reliability required for certifying the marketability of domestic and trade commodities. 
AOAC-Approved for Superior Performance and Fitness-for-Purpose
The Association of Official Analytical Chemists' (AOAC's) validation of VICAM immunoaffinity columns is recognized as the gold standard of approval by quality control programs and regulatory agencies around the world. As a not-for-profit scientific organization dedicated to fostering "worldwide confidence in analytical results," the AOAC plays a leading role in promoting global agreement on the measurement standards and test methods used enforce regulatory compliance and resolve business and trade disputes. The AOAC's choice of immunoaffinity columns for sample cleanup in a growing number of confirmatory analytic methods testifies to the exceptional sensitivity and selectivity of the columns, as well as their simplicity, efficiency, and cost-effectiveness for quantitative applications.
VICAM's AOAC-Validated Test Methods ensure the acceptance of test data throughout the global marketplace:
  • AflaTestTM HPLC: 991.31 Aflatoxin in Corn, Raw Peanuts, and Peanut Butter, Immunoaffinity Column (AflaTestTM) Method
  • AflaOchraTM HPLC: 2008.02 AOAC® International has awarded Official 2008.02 Aflatoxins B1, B2, G1 and G2 and Ochratoxin A in Ginseng and Ginger by Multi-toxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation
The Superior Value of Immunoaffinity Technology continues to gain recognition from international standardization bodies. Officially validated analytic protocols for mycotoxin determination in various foods recently published by European Committee for Standardization (CEN) include the following immunoaffinity-based methods:
  • EN 15829 - Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit, and dried figs: HPLC with immunoaffinity column cleanup and fluorescence detection.
  • EN 15835 - Determination of ochratoxin A in cereal-based foods for infants and young children: HPLC with immunoaffinity column cleanup and fluorescence detection.
  • EN 15850 - Determination of zearalenone in maize-based baby food, barley flour, maize flour, polenta, wheat flour, and cereal- based foods for infants and young children: HPLC with immunoaffinity column cleanup and fluorescence detection.
  • EN 15851 - Determination of aflatoxin B1 in cereal-based foods for infants and young children: HPLC with immunoaffinity column cleanup and fluorescence detection.
  • EN 15891 - Determination of deoxynivalenol in cereals, cereal products, and cereal-based foods for infants and young children: HPLC with immunoaffinity column cleanup and UV detection.
Source
Diego Montemayor, DVM (VICAM)
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