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24th International Pig Veterinary Society Congress (IPVS) 2016
The following technical article is related to the event::
24th International Pig Veterinary Society Congress (IPVS) 2016

Distribution of Toxoplasma gondii in pigs with a high and a low dose of infection

Published on: 10/28/2022
Author/s : M. Cañete-Buenestado 1; R. J. Astorga 2; F. Cardoso-Toset 1, 2; I. M. Rodríguez-Gómez 3; Á. Martínez-Moreno 2; L. Carrasco 3; J. Gomez-Laguna 1, 3 / 1 CICAP - Food Research Center, Pozoblanco; 2 Animal Health; 3 Anatomy and Comparative Pathology, University of Córdoba, Córdoba, Spain.
Summary

Keywords: Distribution, Dose, Toxoplasma gondii.

Introduction:
Toxoplasma gondii continues being one of the main food safety hazards for pregnant women and immunocompromised patients. In this sense, the distribution of this protozoan within the organism and the role of the infective dose in tissue distribution are of highly interest to understand the risk associated to the parasite and to decipher the pathobiology of this infection.
Materials and Methods:
To carry out this study 11 Iberian pigs (twelve-month-old, 100Kg BW, male) were randomly allocated within 3 experimental groups: (1) Control group (2 pigs); (2) High Dose (HD) (5 pigs), animals intramuscularly inoculated with 1x107 tachyzoites of T. gondii strain BCR Reference TgH 00001 (PRU); and (3), Low Dose (LD) (4 pigs), animals intramuscularly inoculated with 1x103 tachyzoites of the same T. gondii strain. Blood samples were collected one week prior to inoculation and at 0, 15 and 30 days post-infection (dpi). At 30dpi all the animals were euthanized and samples from the brain, heart, tongue, lung, liver, spleen, kidney, mesenteric lymph node, masseter muscle and a pool of shoulder, loin and ham (meat pool) were collected at the post-mortem examination. All organs were examined for gross lesions and subjected to qPCR (TOX4-TOX5) to determine the presence of the parasite.
Results:
No changes were detected in sera from control and LD animals throughout the study, but HD animals presented between 3.8 and 16.1 fold increase in the OD (ID Screen ToxopIasmosis, IDvet). No gross lesions were detected in control or infected animals along the study. T. gondii DNA was not detected in control animals. T. gondii DNA was more frequently detected from brain and meat pool samples from HD animals (80% and 80%, respectively) than LD animals (25% and 0%, respectively). On the contrary, T. gondii DNA was more frequently detected from lung, liver and spleen samples from LD animals (75%, 100% and 80%, respectively) than HD animals (40%, 40% and 40%, respectively). No differences were observed in T. gondii distribution between HD animals and LD animals in heart, tongue, masseter muscle, kidney and mesenteric lymph node samples (100%, 40%, 80%, 100% and 100% vs 75%, 50%, 100%, 75% and 75%, respectively).
Conclusion:
Our results highlight that despite the fact that T. gondii might present different tissue tropism according to the infective dose, target skeletal muscles are similarly affected independently of the infective dose. Therefore, different organs should be analysed to clarify the pathogenesis of this infection.
Disclosure of Interest: None Declared.
      
Published in the proceedings of the International Pig Veterinary Society Congress – IPVS2016. For information on the event, past and future editions, check out https://ipvs2024.com/.
 
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