Effect of postbiotic from lactic acid bacteria on the growth of Lactobacillus strains from poultry and swine intestines

Postbiotics produced from lactic acid bacteria consist of a wide range of molecules including peptidoglycans, teichoic acids, exopolysaccharides, cell surface proteins, secreted proteins (protein p40/p75, aggregation-promoting factor and bacteriocins), short-chain fatty acids, conjugated linoleic acid and neurotransmitters which play significant positive roles on the host biological processes such as immunomodulatory, prebiotic, antimicrobial, and gut barrier-preservation effects. Lumensa (AAFCO 36.12 Liquid Lactobacillus acidophilus Fermentation Product) applied to antibiotic-free diets has been shown to improve performance, reduce pathogenic bacteria and increase Firmicutes and Bacteroidetes ratio in cecum of broiler chickens exposed to heat stress. The objective of this study was to evaluate the effect of Lumensa on the growth of Lactobacillus strains from poultry and swine intestines in an in vitro model. Lumensa for Poultry was evaluated for bacterial growth performance of L. reuteri ATCC 55148 (isolated from chicken intestine), L. gallinarum ATCC 33199 (isolated from chicken crop) compared with non-treated control at 37°C under anaerobic condition (5.6% CO₂). Supplementation of Lumensa for Poultry (400 ppm) significantly increased cell density (OD_650nm, P < 0.01), bacterial counts (P < 0.01) and pH change (P < 0.01) after 24 h culture of Lactobacillus strains from poultry intestines compared with control. Lumensa for Swine was evaluated for bacterial growth performance of L. acidophilus ATCC 43121 (isolated from swine rectum), L. reuteri ATCC 53608 (isolated from swine intestine), and L. amylovorus ATCC 33198 (isolated from swine small intestine). Lumensa for Swine (400 ppm) significantly increased cell density (OD_650nm, P < 0.01), bacterial counts (P < 0.01) and pH change (P < 0.01) after 24 h culture of Lactobacillus strains from swine intestines compared with control. In conclusion, supplementation of Lumensa significantly increased the growth of beneficial Lactobacillus strains present in poultry and swine intestines in an in vitro model.

Key Words: Lactobacillus sp., Lumensa, postbiotic.

Presented at the 9th Symposium on Gut Health in Production of Food Animals, St. Louis, USA, 2021. For information on the next edition, click here.