Potential of enzymatically hydrolyzed yeast (AVIATORTM) binding to enterotoxigenic Escherichia coli in vitro

The use of Saccharomyces cerevisiae or enzymatically hydrolyzed yeast (EHY) as a nutritional feed supplement is commonly applied in many livestock productions. It improves animal production, promote health and also reduces the need for antibiotic use. The major component of EHY is mannan oligosaccharide which can improve gut health and act as a high-affinity ligand offering competitive binding site options for Gram negative bacteria.
 
The objective of this study is to evaluate the efficiency of AVIATORTM bind to enterotoxigenic E. coli. The qualitative and quantitative EHY assays were performed in vitro with four enterotoxigenic E. coli strains. The qualitative assay was performed using overnight E. coli culture in TSB broth mix with various EHY concentrations and observed for the agglutination. The EHY-E. coli complex was agglutinated and easily observed in various concentration of EHY as clumping cell. Furthermore, the quantitative assay was also performed by counting the unbound E. coli after EHY reaction. The result showed that EHY has ability to reduce the number of E. coli approximately 3-4 log cells from original cell number. In conclusion, EHY showed the high potential agglutinate and reduce the number of enterotoxigenic E. coli in vitro. This positive phenomenon of EHY will associated with pathogen removal from the gastrointestinal tracts without attachment and colonization in vivo.
 
Keywords: Aviator, Escherichia coli, ETEC, enzymatically hydrolyzed yeast, agglutination.

Abstract presented at the 3rd International Symposium on Alternatives to Antibiotics 2019.