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Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet

Published: March 11, 2020
By: A. M. Abdelhamid 1, H. R. Behery 2 and Sahar R. M. Hammoudah 1. / 1 Department of Animal Production, Faculty of Agriculture, Mansoura University, Egypt; 2 El-Serw Animal Breeding Station, Animal Research Institute, Agricultural Research Center, Egypt.
Summary

An experiment was conducted on newly weaned rabbits for eight weeks by fattening them on diets supplemented with corn steep liquor (CSL) replaced 0, 10, 20, and 30% of crude protein and energy of barley and soybean meal of the diets. The obtained results refer to increased feed intake (thus, worst the feed conversion and economic efficiency); serum cholesterol, triglycerides, and low density lipoproteins (referring to somewhat negative effect on liver function, where the serum alanine aminotransferase activity was decreased too); as well as hemoglobin concentration and red blood cells count (as good indicators). Otherwise, there were no significant effects on growth performance, water intake, edible parts, digestibility, and muscular composition. So, it could be concluded that dietary inclusion of CSL (up to 30%) for rabbits is possible without adverse effects on performance and health state.

Introduction
Searching for novel economic animal food sources to overcome the Egyptian gap in animal nutrition, many attempts were carried out to evaluate new agro-industrial by-products (Abdelhamid, 1988) such as rice straw and maize stover (El-Shinnawy et al., 1986 and Abdelhamid et al., 1989a, b, 1991 &1994), poor quality barley straw (Gabr et al., 1989), pea by-products (Abdelhamid and El-Ayoty, 1988), dried sugar beet pulp (Abdelhamid, 1992), whole sunflower seeds (Abdelhamid et al., 2011a; 2012 & 2013a & b) and sieving wastes of Egyptian clover's seeds (Abdelhamid and Saleh, 2015 and Abdelhamid et al., 2016). Different field crops and wastes such as grass silages (Abdelhamid and Topps, 1991), and new sources of fodder (clitoria and phillipesara, Abdelhamid and Gabr, 1993 as well as teosinte and kochia, Shehata et al., 2001 and Ahmed et al., 2001) were evaluated too. Agricultural by-products (Abdelhamid et al., 2009a & b) as banana waste (Abdelhamid et al., 2009c & d) and aquatic and salt plants as water hyacinth (Abdelhamid and Gabr, 1991a & b and Abdelhamid et al., 2006 & 2007) were studied also. Besides animal wastes as poultry litter (Gabr et al., 1991a & b) as unconventional feedstuffs (Abdelhamid et al., 1992 & 2001a & b and Abdelhamid, 2004) or unconventional silage making using plant and animal wastes (Abdelhamid et al., 2001a & b). Feed additives as chamomile flowers (Abdelhamid et al., 2004a & b) and other medical herbs (Abdelhamid et al., 2011b) and biologically treated diets (Abdel-Khalek et al., 2012) were fed to different animal species. Also, many other feed additives were used in animal nutrition including propylene glycol (McClanahan et al., 1998 and Nielsen and Ingvartsen, 2004) and calcium propionate (Abdel-Latif et al., 2016 and Gabr et al., 2017). Therefore we attempt herein to evaluate the possibility of using corn steep liquor (CSL) in rabbits' diet.
Materials and methods
Thirty six male and female "Balady" local rabbits strain, directly after weaning (ca. 28 days old), were purchased from the local market then divided into four groups; each (of eight males plus one female rabbits) was divided into three subgroups, each of three rabbits of initial live bodyweight of 602 – 611 grams. The experimental groups were:
  • The first one was considered as a control group, its rabbits were fed a commercial fattening diet (17% CP).
  • The second group's rabbits were fed the commercial diet with 10% replacement of the total dietary CP (17%) from barley and soybean meal with CSL.
  • The third group's rabbits were fed the commercial diet with 20% replacement of the total dietary CP (17%) from barley and soybean meal with CSL.
  • The fourth group's rabbits were fed the commercial diet with 30% replacement of the total dietary CP (17%) from barley and soybean meal with CSL.
The CSL is obtained from Starch and Glucose Factory, Cairo, Mostorod. It is a by-product of wet milling process of maize-starch industry. It is a dark yellow flowing liquid with molasses. It contains high levels of soluble protein, glucose and minerals that make it useful to compensate the poor value of low quality forages by increasing the energy and protein levels without more fiber intake. The CSL is a thick liquid contains ca. 57% moisture and 43% dry matter (DM). It contains 33.5% CP on DM basis. It was preserved via concentration to 88% DM and to be similar to the other dietary ingredients. The CSL could be also mixed with corn milling process (corn bran) to avoid further drying into a moist friable mass. The product is gravitationally and microbial stable and is a non-agglomerating mixture having no obvious undesirable wet characteristics. Consequently, the product may be readily transported via conventional transfer systems from its storage location to place of consumption. Since, all animal feeds, whether fluid feeds or non-pelleted solid feeds are prone to component separation especially during distribution and, in the case of high moisture products as are the present products, during storage, this being caused at least in part because of the influence of gravity. In fluid feeds suspended solids tend to precipitate out and in solid feeds liquid components may separate from solid components and various solid components (Linton and Hussar, 1989).
The partial replacement of CSL instead of soybean meal and barley based on crude protein and energy contents. So, four experimental diets were formulated to be iso-caloric and iso-nitrogenous. After concentrating the CSL, all ingredients were mixed then pelleted into 12 mm length and 4 mm diameter of the pellets using chicken and rabbit's feed pelleting Chinese machine with a capacity of 200 Kg / h imported by Kitman Company for Agricultural Tools. The following Table No. 1 presents the chemical composition of CSL as produced (43% DM) and after its concentrating to 88% DM. 
Table 1: Chemical composition (%) of the CSL as purchased (43% DM) and after concentrating (88% DM)
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 1 
Tables 2 and 3 show the formulation and calculated chemical composition of the experimental diets. All rabbits were housed in wire batteries provided with feeders and drinkers under the same environmental conditions concerning air temperature and relative humidity, using thermometer and hygrometer. At the latest five days of the experimental period (eight weeks, after one week for adaptation), feed intake and feces excreted for individual three males /treatment were quantitatively weighed. Collective feed and feces samples were taken for chemical analysis and digestibility calculation. At the end of the feeding experiment and digestibility trial, three male rabbits/treatment were fasted for 12 hours, slaughtered, blood samples collected for complete blood picture, skinned, and portioned to calculate edible parts as well as sampled for chemical analysis of rabbits' meat.
Table 2: The formulation1 of 100 Kg of each experimental diet
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 2 
Table 3: Calculated chemical composition of the experimental diets
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 3 
Digestibility trials: At the collection period of the digestibility trial, 3 male rabbits/treatment group were individually housed in the same batteries, daily feed consumption and feces excreted were weighed, samples were taken and kept in a refrigerator at – 4 ºC till the chemical analysis was undertaken.
Slaughter test: Three rabbits/group were fasted for 12 h then weighed, sacrificed, blood samples collected, de-skinned, different parts separated and weighed, and trunk meat samples collected.
Chemical analysis: Dry matter, crude protein, ether extract, and/or ash of feeds, feces and meat samples were analyzed using FOSS NIRS TM DA 1650, Denmark.
Hematological parameters: Hematological parameters including count of red blood cells (RBC's) and white blood cells (WBC's), packed cell volume (PCV%), and hemoglobin concentration were counted or measured in fresh whole blood drawn into heparinized test tubes using fully digital hematology counter (Laboratories, USA).
Blood serum analysis: Other collected samples were allowed to clot and centrifuged at 3500 rpm for 20 minutes to separate blood serum. Serum was carefully decanted into labeled tubes using serological pipettes and stored at -20 ºC until analysis. Where total protein and albumin concentrations were determined using commercial kits according to the Douman et al. (1971). Globulin was calculated by difference. Using commercial kits purchased from bio-Merieux, Laboratory Reagents and Products, France, creatinine was estimated in serum by the method of Joffe reaction described by Giorgio (1974) with standard creatinine purchased from Boehringer Mannheim Gmb H-W Germany. Activities of serum transaminases AST and ALT were determined according to Reitman and Frankel (1957) using a colorimetric method via commercial kits. Blood serum was tested also for uric acid, cholesterol, triglycerides, and high density lipoprotein (HDL) concentrations using commercial kits. The low density lipoprotein (LDL, the bad cholesterol) concentration was calculated by subtracting the high density lipoprotein (HDL, the good cholesterol) concentration and triglycerides concentration (divided by 5) from the total cholesterol concentration [LDL = total cholesterol – HDL – (triglycerides / 5)].
Statistical analysis: The obtained numerical data were statistically analyzed using standard error (SE), coefficient of variance (CV % = 100 [S / mean (?) ] ), and statistical analysis system software (SAS, 2006) for windows. One way analysis of variance and (Duncan, 1955) multiple range tests were used to compare between the parameters of the different nutritional group. The differences were significant at 0.05 levels.
Results
Feed and water consumption: The daily feed consumption of the experimented rabbits ranged between 88.89 ± 6.41and 133.3 ± 0.64 g/h/d with increasing trend by age going on (from the 1st to the 8th week of the feeding trial) and with significant (P≤0.05) increases in the case of dietary inclusion of CSL, particularly at 30 and 20% of the diet (Table 4).
Table 4: Feed consumption as means (of 9 rabbits) ± standards errors of the 4 treatment groups (gram/head/day) throughout the experimental period (8 weeks)
 Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 4
Daily water intake (ml/head/day) take the same trend of the daily feed consumption, since it take the range from 38.90 ± 1.67 to 141.6 ± 0.81 with increasing trend by age going on (from the 1st to the 8th week of the feeding trial) and with significant (P≤0.05) increases in the case of dietary inclusion of CSL, particularly during the 2nd and 3rd weeks (Table 5).

Table 5: Drinking water consumption as means (of 9 rabbits) ± standards errors of the 4 treatment groups (ml/head/day) throughout the experimental period (8 weeks)
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 5 
Growth performance: Although the significant (P≤0.05) increases in feed intake by the dietary inclusion of CSL; yet, there were no significant (P≥0.05) differences among treatments in live bodyweight (Table 6) throughout the experimental intervals (8 weeks).
Table 6: Live bodyweight in grams as means (of 9 rabbits) ± standards errors of the 4 treatment groups throughout the experimental period (8 weeks)
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 6
Nutrients digestibility: Table 7 presents means ± standard errors of digestibility coefficiency of different nutrients by the experimented rabbits at the end of the fattening period. There were no significant (P≥0.05) differences among treatments in the dry and organic matters digestibility. Yet, there were significant (P≤0.05) differences in the crude protein digestibility (since G2 was the best) and ether extract digestibility (since G4 was the worst whereas G1 was the best). 
Table 7: Means ± standard errors of digestibility coefficiency of different nutrients (%, dry matter basis)
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 7 
Feed utilization:Feed conversion ratio and economic efficiency of feeding rabbits with CSL-including diets during the fattening period of 8 weeks (Table 8) showed that the best diet was the control one, since CSL-inclusion increased the feed intake (Tables 4 and 8) and did not improve body weight (Tables 6) nor body weight gain (Table 8).
Table 8: Economic efficiency of feeding rabbits with CSL-including diets during the fattening period as means (of 9 rabbits) ± standards errors of the 4 treatment groups
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 8 
Slaughter test: Absolute weights (g) of different edible parts of the experimental rabbits after the fattening period (8 weeks) as means (of 3 rabbits) ± standards errors of the 4 treatment groups are given in Table 9. This Table reflects no significant (P≥0.05) differences among treatments at the end of the experiment. However, the chemical analysis of the carcass meat revealed no remarkable differences among different treatments; since the dry matter contents were 71.1, 71.4, 73.1, and 71.7% and crude protein contents were 80.6 ± 0.23, 79.8 ± 1.55, 70.2 ± 1.59, and 81.5 ± 0.26% for G1, G2, G3, and G4, respectively.
Table 9: Absolute weights (g) of different edible parts of the experimental rabbits after the fattening period (8 weeks) as means (of 3 rabbits) ± standards errors of the 4 treatment groups
 Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 9
Table 10: Relative weights (% of the live body weight) of different edible parts of the experimental rabbits after the fattening period (8 weeks) as means (of 3 rabbits) ± standards errors of the 4 treatment groups 
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 10 
Blood biochemical parameters: Table 10 presents means ± standard errors and variation coefficient of some biochemical parameters determined in rabbits' blood sera of different dietary treatments. The significant (P≤0.05) effects were calculated among treatments only for ALT activity, and concentrations of creatinine, uric acid, cholesterol, triglyceride, and LDL. The best values were obtained by the first treatment (10 % replacement) concerning ALT, creatinine, uric acid, cholesterol, and LDL. The higher replacement percentages (20 and 30 %) reduced ALT activity, and increased the concentrations of creatinine, cholesterol, and LDL. That means that the replacement in rabbits' diets must be not exceeding 10 % to maintain the public health [liver function (via lowering the activity of ALT and increasing the concentration of cholesterol and triglyceride) and heart and blood vessels (through thrombosis via increasing LDL levels)] of the treated rabbits. Yet, the obtained values lying within the normal ranges of the biochemical measurements for rabbits' sera.
Table 10: Effect of the dietary treatments on the blood biochemical parameters* of the tested rabbits at the end of the experiment (means ± standard errors and variation coefficient (V %) between brackets)
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 11 
Blood hematological parameters: Table 11 presents means ± standard errors and variation coefficient of some hematological parameters determined in rabbits' blood of different dietary treatments. The significant effects were calculated among treatments only for granulocytes count and percentage, lymphocytes percentage, hemoglobin concentration, red blood cells' count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cells distribution width-standard deviation, platelets count, and platelet crit. The higher replacement percentages (20 and / or 30 %) elevated significantly (P≤0.05) the granulocytes count and %, lymphocytes % (referring to higher immunity), hemoglobin level, red blood cells count, hematocrit %, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelets count. That means that the replacement at its high rates (20 and / or 30 %) may improve most of the tested hematological parameters, reflecting an improvement in the public health of the treated rabbits. Yet, the obtained values lying within the normal ranges of the hematological parameters for rabbits.   
Table 11: Effect of the dietary treatments on the blood hematological parameters* of the tested rabbits at the end of the experiment (means ± standard errors and variation coefficient (V %) between brackets)
 Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 12
Possible Using Corn Steep Liquor (CSL) in Rabbits' Diet - Image 13
Discussion
Most of the obtained results herein lying within the normal ranges mentioned for different rabbits' strains by different researchers such as Abdelhamid et al. (2016) for mean daily body weight gain, feed and water daily consumption, feed conversion ratio, digestibility, slaughter test, muscles composition, and blood analysis. Said (2016) concerning feed and water consumption, body weight, feed conversion ratio, edible parts, digestibility, and blood picture. Similar results were registered also by Abdel-Khalek et al. (2012), Sadek (2011), Selim et al. (2012), Abu El-Hamd et al. (2013), El-Medany et al. (2013), Ragab et al. (2013) and Abdelhamid and Saleh (2015) concerning rabbits' performance, digestibility, slaughter test, muscular composition, and blood picture. However, CSL was used as unconventional animal feed source of energy and / or protein for feeding finishing steers (Trenkle, 2002), lambs (Mirza and Mushtaq, 2006; Freitas et al., 2015, and Azizi-Shotorkhoft et al., 2016), Labeo rohita fingerlings and carp (Chovatiya et al., 2010), chicken (Rafhan Product Reference Guide, 2010 and Ullah et al., 2017), lactating cows (Santos et al., 2012), crossbred calves (Siverson, 2013), Rahmani lambs (El-Emam et al., 2014), ewes (Hafez et al., 2015 and Khalifa et al., 2015b) Zaraibi nanny goats (Khalifa et al., 2015a and Saba et al., 2015).
Although the significant effects on some biochemical and hematological parameters; yet, the obtained values lying within the normal ranges of the biochemical measurements for rabbits' sera and the hematological parameters for rabbits according to Merck (1976), who added that Hb concentration and RBCs count increase by actual polyglobulinemia followed by O2 – shortage or dehydration, whereas WBCs count increases by acidosis. However, Merck (1974) mentioned that serum ALT activity increases and quickly decreases by hepatic toxicity, serum uric acid level decreases by acute hepatic dystrophy, whereas serum triglyceride concentration increases by essential hyperlipidemia and hepatic cirrhosis. Moreover, Varley (1978) cited that hypercholesterolemia is found in nephritis. Gout is an inflammatory disease diagnosed by hyperuricaemia. The liver is the most important area for the production of cholesterol (Goldberg, 1999). Hypouricaemia (low serum urate) may arise in severe liver disease, increased excretion, rasburicase (Beckett et al., 2010 and Walker et al., 2013). Hypertriglyceridemia is a risk factor for cardiovascular disease. Deposition of lipids in arterial walls and the subsequent formation of an atheroma are key features of atherogenesis and coronary heart disease (Ahmed, 2011).
Conclusively
From the aforementioned results, it could be concluded that the dietary inclusion of CSL in rabbits' diets (up to 30 % of crude protein and energy of dietary barley and soybean meal) could be beneficial in case of barley and soybean meal shortage, unavailable or tend to be expensive. However, the control diet was better concerning feed conversion and economic efficiency (cost of feeding to produce one kilogram body weight gain). The CSL-inclusion improved to some extent the blood parameters and did not negatively affect the rabbit's performance.   

  • Abdelhamid, A. M. (1988). Utilization from Agro-industrial By-products in Ruminant’s Feeding. 1st Nat. Conf. On the Role of the Sci. Res. in Raising Animal Wealth. 2nd Forum: Developing Ruminant’s Feed Resources. 25-29 Sept., pp: 119-130. Acad. Sci. Res. Technol., Cairo.
  • Abdelhamid, A. M. (1992). Feeding value of dried sugar beet pulp from Egyptian production. Arch. Anim. Nutr., 42: 365-370.
  • Abdelhamid, A. M. (2004). Illegality of some unconventional manufactured feeds for various animals. Proc. Sci. Con.”Husbandry and Development of Animal Wealth in Islamic Civilization and Today’s Systems”. 28 Feb. - 1 Mar., Al-Azhar Univ., 48 p.
  • Abdelhamid, A. M. and El-Ayoty, S. A. (1988). Feeding sheep on pea by-products produced during preparation for freez-preservation. Archiv für Tierernährung, 38: 789-797 38: 757-766.
  • Abdelhamid, A. M. and Gabr, A. A. (1991a). Evaluation of water hyacinth as a feed for ruminants. Arch. Anim. Nutr., Berlin, 41: 745-756.
  • Abdelhamid, A. M. and Gabr, A. A. (1991b). Utilization of water-hyacinth hay in comparison with berseem hay as sole feeds by sheep with emphasis on its hazardous effects. J. Agric. Sci. Mansoura Univ., 16: 507-517.
  • Abdelhamid, A. M. and Gabr, A. A. (1993). The evaluation of new sources of fodder (clitoria and phillipesara) under Egyptian conditions. Arch. Anim. Nutr., 44: 85-93.
  • Abdelhamid, A.M. and Saleh, M.T.M. (2015). Evaluation of substituting the sieving wastes of Egyptian clover's seeds instead of soya bean in the diet of Flan-line rabbits. J. Animal and Poultry Prod., Mansoura Univ., 6 (3): 137-147.
  • Abdelhamid, A. M. and Topps, J. H. (1991). Effect of a dietary concentrate on the digestibility of grass silages. Arch. Anim. Nutr., Berlin, 41: 737-744.
  • Abdelhamid, A. M.; Abdel-Khalek, A. E., Ashmawy, T. A. M. Abou Ammou, F. F. and El-Sanafawy, H. A. (2013a). Effect of dietary inclusion of whole sunflower seeds on feeding lactating Zaraibi goats: II. On milk production and composition as well as mammary gland histology and economic efficiency. International Journal of Biotechnology Research, 1 (1): 6-19.
  • Abdelhamid, A. M.; Abdel-Khalek, A. E., Ashmawy, T. A. M., Abou Ammou, F. F. and El-Sanafawy, H. A. (2013b). Effect of dietary inclusion of whole sunflower seeds on feeding lactating Zaraibi goats: IV. On growth and reproductive performance of their kids. Int. J. Food Nutr. Saf., 3 (1): 127-145.
  • Abdelhamid, A. M.; Abdel-Khalek, A. E., Ashmawy, T. A. M., Abou Ammou, F. F. and El-Sanafawy, H. A. (2012). Effect of dietary inclusion of whole sunflower seeds on feeding lactating Zaraibi goats: III. On their blood profile.
  • Abdelhamid, A. M.; Abdel-Khalek, A. E., Ashmawy, T. M., Abou Ammou, F. M. and El-Sanafawy, H. A. (2011a). Effect of dietary inclusion of whole sunflower seeds on feeding lactating Zaraibi goats: I. on digestibility coefficients, rumen function and live body weight digestibility coefficients, rumen function and live body weight. J. Animal and Poultry Production, Mansoura University, 2: 535-547
  • Abdelhamid, A. M.; Abou Ammou, F. F., Abdel-Khalek, A. E., Ahmed, M. E., E. I. Shehata, M. E. and Maged, G. A. (2004a). Effect of dietary supplementation with chamomile flowers on carcass characteristics and histology of some organs in Rahmani sheep. J. Agric. Sci. Mansoura Univ., 29: 6119 – 6135.
  • Abdelhamid, A. M.; Ahmed, M. E., Shehata, E.I., Abou Ammou, F. F. and Maged, G. A. (2004b). Impact of using chamomile flowers on the performance of Rahmani sheep. J. Agric. Sci. Mansoura Univ., 29: 6105 – 6117.
  • Abdelhamid, A. M.; Bassuny, S. M., Abd El-Aziz, A. A. and Ibrahim, M. Y. S. A. (2009a). Evaluation of biological treatments for agricultural by-products in ruminants feeding. II- Digestibility study. J. Agric. Sci. Mansoura Univ., 34: 6239 – 6250.
  • Abdelhamid, A. M.; Bassuny, S. M., Abd El-Aziz, A. A. and Ibrahim, M. Y. S. A. (2009b). Evaluation of biological treatments for agricultural by-products in ruminants feeding. III- Growth of lambs. J. Agric. Sci. Mansoura Univ., 34: 6251 – 6259.
  • Abdelhamid, A. M.; El-Ayoty, S. A., Topps, J. H., El-Shinnawy, M. M., Gabr, A. A. and El-Sadaney, H. H. (1992). Evaluation of some unconventional and conventional feeds in Dakahlia Governorate. Arch. Anim. Nutr., 42: 371-381.
  • Abdelhamid, A. M.; El-Shinnawy, M. M. and El-Emam, G. I. I. (1991). Subsidizing rice straw with urea for partial substitution of concentrate feed mixture in fattening diets of crossbred calves. J. Agric. Sci. Mansoura Univ., 16: 1511-1523.
  • Abdelhamid, A. M.; El-Shinnawy, M. M., Gabr, A. A., Topps, J. H. and Abou Raya, A. K. (1989b). Urea as a source of ammonia for improving the nutritive value of rice straw and maize stover. 2- In sacco and in vivo evaluation. J. Agric. Sci. Mansoura Univ. 14: 1530-1542.
  • Abdelhamid, A. M.; Fayed, A. M., Ghanem, A. Z. and Helal, H. G. (2007). Studies on biological treatment of salt plants. II– Fattening trial. J. Agric. Sci. Mansoura Univ., 32: 151 – 165.
  • Abdelhamid, A. M.; Fayed, A. M., Ghanem, A. Z. and Helal, H. G. (2006). Studies on biological treatment of salt plants. 1- Feed evaluation by small ruminants. J. Agric. Sci. Mansoura Univ., 31: 627 – 640
  • Abdelhamid, A. M.; Gabr, A. A. and. El-Shinnawy, M. M. (1994). Effect of hydrogen peroxide and urea treatment on chemical composition, cell wall constituents and in vitro organic matter digestibility of rice straw and maize stover. J. Agric. Sci. Mansoura Univ., 19: 3647-3657
  • Abdelhamid, A. M.; Gabr, A. A., El-Shinnawy, M. M., Topps, J. H. and Abou Raya, A. K. (1989a). Effect of anhydrous ammonia treatment on improving the nutritive value of rice straw and maize stover. J. Agric. Sci. Mansoura Univ., 14: 1504-1518.
  • Abdelhamid, A. M.; Ghanem, G. H. A., Aiad, A. M. and Matari, R. I. M. (2009c). Evaluating the possibility of recycling banana waste as a feed for ruminants. I- chemical composition, rumen-liquor parameters, digestibility coefficients, and feeing values by lambs. J. Agric. Sci. Mansoura Univ., 34: 10451 – 10467.
  • Abdelhamid, A. M.; Ghanem, G. H. A., Aiad, A. M. and Matari, R. I. M. (2009d). Evaluating the possibility of recycling banana waste as a feed for ruminants. II- growth performance, blood picture, and feeding economics by lambs. J. Agric. Sci. Mansoura Univ., 34: 10469 – 10479.
  • Abdelhamid, A.M.; Ismail, R. F. S. A. and Saleh, M. T. M. (2016). Evaluation of complete substitution of sieving wastes of the Egyptian clover seeds instead of soybean meal and maize in rabbit's diet. J. Animal and Poultry Prod., Mansoura Univ., 7 (5): 153-162.
  • Abdelhamid, A. M.; Nowar, M. S., Bassuny, S. M. and El-Emam, G. I. (2001a). Evaluation of unconventional silage making using plant and animal wastes in feeding ruminants. J. Agric. Sci. Mansoura Univ., 26: 5349 – 5360.
  • Abdelhamid, A. M.; Shehata, E. I., Esa, H. R. B., Gomaa, I. A. and Abd Allah, G. A. (2001b). Possible side effects of unconventional feeding of livestock animals. J. Agric. Sci. Mansoura Univ., 26: 5371 – 5380.
  • Abdelhamid, A. M.; Shehata, E. I. and Maged, G. A. (2011b). Effect of some medical herbs on production of dairy Zaraibi goats. J. Animal and Poultry Production, Mansoura University, 2: 493-513.
  • Abdel-Khalek, A. E.; Abdelhamid, A. M., Mehrez, A. F. and El-Sawy, I. (2012). Growth performance, digestibility coefficients, blood parameters and carcass traits of rabbits fed biologically treated diets. J. Animal and Poultry Production, Mansoura University, 3: 227-239.
  • Abdel-Latif, M.A.; EL-Gohary, E.S., Gabr, A.A., El-Hawary, A.F., Ahmed, S.A., Ebrahim, S.A. and Fathala, M.M. (2016). Impact of supplementing propylene glycol and calcium propionate to Primiparous buffalo cows during the late gestation and early lactation period on reproductive performance and metabolic parameters. Alexandria Journal of Veterinary Sciences, 51 (1): 114-121.
  • Abu El-Hamd, M. A., Sheteifa, M. A. M. and Ragab, A. A. (2013). Effect of ascorbic acid on performance and reproductive performance of does New Zealand white rabbit. J. Animal and Poultry Prod., Mansoura Univ., 4 (9): 549-559.
  • Ahmed, M. A.; Abdelhamid, A. M., Abou Ammou, F. F., Soliman, E. S., El-Kholy, N. M. and Shehata, E. I. (2001). Response of milk production of Zaraibi goat to feeding silage containing different levels of teosinte and kochia. Egypt. J. Nutr. and Feeds, 4: 141 – 153.
  • Ahmed, N. (2011). Clinical Biochemistry, Oxford University Press Inc., New York. ISBN 978–0–19–953393–0.
  • Azizi-Shotorkhoft, A.; Sharifi, A., Mirmohammadi, D., Baluch-Gharaei, H. and Rezaei, J. (2016). Effects of feeding different levels of corn steep liquor on the performance of fattening lambs. Journal of Animal Physiology and Animal Nutrition, 100: 109–117.
  • Beckett, G.; Walker, S., Rae, P. and Ashby, P. (2010). Lecture Notes: Clinical Biochemistry, Eighth Edition, A John Wiley & Sons, Ltd., Publication, Printed in Malaysia.
  • Chovatiya S. G.; Bhatt S. S. and Shah A. R. (2010). Evaluation of raw and Corn Steep Liquor as a supplementary feed for Labeo rohita (Ham) fingerlings. Aquaculture International, DOI 10.1007/s10499-010-9336-5. Ph.D. Thesis; BRD School of Biosciences, Sardar Patel University.
  • Douman, B.T.; Waston, W.A. and Homer, G.B. (1971). Albumin standards and the measurement of serum albumin with bromo-cresol green. Clinic. chemical Acta, 31: 87 – 90.
  • Duncan, D.B. (1955). Multiple ranges and multiple F-tests. Biometrics, 11: 1-42.
  • El-Emam, G. I.; Hafez, Y.H., Behery, H.R., Khalifa, E.I., Shehata, E. I. and Ahmed, M. E. (2014). Growth performance, some rumen and blood parameters of growing Rahmani lambs fed rations containing triticale or berseem silages and their mixture. Egyptian Journal of Sheep & Goat Sciences, 9 (1): 67- 76.
  • El-Medany, Sh.A.; El-Reffaei, W.H. and Nada, S.A. (2013). Effect of different oils on growth performance and carcass traits in growing rabbits. J. Animal and Poultry Prod., Mansoura Univ., 4 (12): 733-745.
  • El-Shinnawy, M. M.; Abdelhamid, A. M., Abu Raya, A. K. and Gabr, A. A. (1986). Recent development for improving the feeding quality of rice straw suiting various conditions. Third International Rice Conference, Alexandria, 11 pp.
  • Freitas, A.P.D. de; Ferreira, M. deA., Oliveira, J.P.F. de, Silva, Á.E.M. da, Soares, L.F.P., Silva, J.deL., Salla, L.E. and Souza, A.R.D.L. (2015). Replacement of soybean meal with maize steep liquor in the diets of feedlot lambs. South African Journal of Animal Science, 45 (5).
  • Gabr, A. A.; Abdelhamid, A. M., El-Ayek, M. Y. and Mehrz, A. Z. (1991a). Substituting concentrate feed mixture by dried poultry litter and molasses in rations of sheep containing low quality agricultural residues. J. Agric. Sci. Mansoura Univ., 16: 1704-1714.
  • Gabr, A. A.; Abdelhamid, A. M. and El-Ayek, M. Y. (1991b). Nutritional evaluation of dried poultry litter in comparison with berseem hay (Trifolium alexandrinum) and their mixtures as feed for sheep. J. Agric. Sci. Mansoura Univ., 16: 2004-2016.
  • Gabr, A. A.; Topps, J. H., El-Shinnawy, M. M. and Abdelhamid, A. M. (1989). The productive value of poor quality barley straw treated with ammonia and comparison of methods to assess the improvements in quality. 3rd Egypt. British Conf. on Animal, Fish and Poultry Production. Alexandria, 7 - 10 October, pp: 271-280.
  • Gabr, A.A.A.; Ebrahim, S.A., El-Hawary, A.F.A., Fathala, M.M., EL-Gohary, E.S.H., Ahmed, S.M. and Abdel-Latif, M.A. (2017). Upgrading milk productivity of Primiparous buffaloes using glycogenic precursors; implications on milk production and blood biochemical parameters. Zagazig Veterinary Journal, 45 (2): 92-103.
  • Giorgio, J.D. (1974). Creatinin estimation: Clinical chemistry principles and techniques. Henry et al., eds., pp: 242 – 543 Harper and Row, Hagerstown.
  • Goldberg, S. (1999). Clinical Biochemistry Made Ridicuwusly Simple, 2nd Ed., Ninth Printing. MedMaster, Inc., Miami, ISBN #0-940780-30-5.
  • Hafez, Y. H.; Khalifa, E. I., Behery, H. R., Mahrous, A. A., Fayed, A.M.A. and Hassanien, H.A. M. (2015). Productive and reproductive performance of ewes and growth rate of lambs as affected by non-conventional energy supplement to rations. Egyptian Journal of Sheep & Goat Sciences, 10 (2): 81- 93.
  • Khalifa, E. I., Behery, H. R., Hafez, Y. H. , Mahrous, A. A., Fayed, A.A. and Hassanien, H.A.M. (2015a). Supplementing non-conventional energy sources to rations for improving production and reproduction performances of dairy Zaraibi Nanny goats. Egypt. J. of Sheep & Goat Sciences, 10 (2): 81-93.
  • Khalifa, E. I.; Hafez, Y. H., Mahrous, A. A., Behery , H. R., Hassanien, H. A. M. and Fayed, A. A. (2015b). Impact of non-conventional energy sources in ration on productive and reproductive performance of ewes. Egyptian Journal of Sheep & Goat Sciences, Proceedings Book of the 5th International Scientific Conference on Small Ruminant Production, Sharm El Sheikh-Egypt, P: 53-65.
  • Linton; J.H. and Hussar, N. (1989). Animal feed supplement prepared from wet corn bran and corn steep liquor. United States Patent No. 1191, 4,859,485.
  • McClanahan, S., Hunter, J., Murphy, M. and Valberg, S. (1998). Propylene glycol toxicosis in a mare. Veterinary and Human Toxicology, 40: 294–296.
  • Merck, E. (1974). Klinisches Labor. 12. Auflage, E. Merck, Darmstadt, Deutschland.
  • Merck, E. (1976). Labordiagnostik in der Tiermedizin. Diagnostica Merck, Deutschland.
  • Mirza, M.A. and Mushtaq, T. (2006). Effect of supplementing different levels of corn steep liquor on the post-weaning growth performance of Pak-Karakul lambs. Pakistan Vet. J., 26 (3): 135-137.
  • Nielsen, N.I. and Ingvartsen, K.L. (2004). Propylene glycol for dairy cows: a review of the metabolism of propylene glycol and its effects on physiological parameters, feed intake, milk production and risk of ketosis. Animal Feed Science and Technology, 115: 191–213.
  • NRC (National Research Council) (1977). Nutrient Requirements. National Academy Press. Washington. D. C., USA.
  • Rafhan Product Reference Guide. (2010). Lazofertn™, animal feed ingredient. Faisalabad: Rafhan Maize Products.
  • Ragab, A.A.; El-Reidy, K.F.A. and Gaafar, H.M.A. (2013). Effect of diet supplementation with pumpkin (Cucurbita moschata) and black seed (Nigella sativa) oils on performance of rabbits: 1- Growth performance, blood hematology and carcass traits of growing rabbits. J. Animal and Poultry Prod., Mansoura Univ., 4 (7): 381-393.
  • Reitman, S. and Frankel, S. (1957). Colorimetric GOT and GPT Transaminases determination. Amer. J. Clin. Path., 28: 57 – 63.
  • Saba, F. E.; Behery, H.R., Gomaa, A.A.A.I., Abdel-Gawad, A. M. and Ahmed, M.E. (2015). Effect of corn steep liquor on performance of dairy Zaraibi goats. Egyptian Journal of Sheep & Goat Sciences, Proceedings Book of the 5th International Scientific Conference on Small Ruminant Production, Sharm El Sheikh-Egypt, P: 43-52.
  • Sadek, A.M.A. (2011). Studies in feeding rabbits. Ph.D. Thesis, Faculty of Agriculture, Mansoura University.
  • Said, M.M.A. (2016). Effect of some medicinal herbs on rabbits performance. M.Sc. Thesis, Animal Production, Faculty of Agriculture, Zagazig University.
  • Santos, V. L. F. D.; Ferreira, M. D. A., Guim, A., Silva, F. M. D., Urbano, S. A. and Silva, E. C. D. (2012). Protein sources for crossbred dairy cows in the semiarid. R. Bras. Zootec., 41 (10): 2272-2278.
  • SAS (2006). SAS/STAT Guide for personal computer. SAS Inst. Cary, N. C.
  • Selim, N., Abdel-Khalek, A. M. and Gad, S. M. (2012). Effect of supplemental zinc, magnesium or iron on performance and some physiological traits of growing rabbits. Asian Journal of Poultry Science, 6 (1): 23-30.
  • Shehata, E. I.; Ahmed, M. E., Abdelhamid, A. M., Abou Ammou, F. F. and El- Haggag, M. (2001). Comparative nutritive values of silage rations containing different levels of teosinte and kochia. Egypt. J. Nutr. and Feeds, 4: 129 – 140.
  • Siverson, A. (2013). Effects of corn processing and dietary wet corn gluten feed on newly received and growing cattle. Kansas State University, M.Sc. Thesis, College of Agriculture.
  • Trenkle, A. (2002). Relative Feeding Value of Wet Corn Steep Liquor When Fed to Finishing Cattle. A.S. Leaflet R1773, Beef Research Report — Iowa State University.
  • Ullah, Z.; Yousaf, M., Shami, M. M., Sharif, M. and Mahrose, Kh. (2017). Effect of graded levels of dietary corn steep liquor on growth performance, nutrient digestibility, haematology and histopathology of broilers. Journal of Animal Physiology and Animal Nutrition. ORCID:orcid.org/0000-0002-9917-5921.
  • Varley, H. (1978). Practical Clinical Biochemistry, 4th Ed. Reprinted, Arnold-Heinemann Publishers (India) Private Limited.
  • Walker, S.; Beckett, G., Rae, P. and Ashby, P. (2013). Clinical Biochemistry, Lecture Notes, Ninth Edition, A John Wiley & Sons, Ltd. Publication, New Delhi, India.
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A.M. Abdelhamid
Mansoura University, Egypt
Mansoura University, Egypt
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