Table 1. Some good silage management practices. | ||
Silage practice | Reasoning | |
• Harvest crop at correct maturity and DM | • Optimizes nutritive value (protein, fiber, energy, etc.) | |
• Corn silage: 1/2 to 2/3 milk line; 35% DM | • In some cases optimizes DM content | |
• Alfalfa: < 1/10 bloom;bunk or bag silo 35 to 45% DM, conventional upright 35 to 50% DM, oxygen limiting silo 45 to 60% DM | • Ensures good packing, elimination of excess oxygen | |
• Grasses: boot; bunk or bag silo - 35 to 45% DM | • Minimizes seepage losses | |
Small grains: boot to dough; 30 to 40% DM | • Prevents clostridial (butyric) fermentation | |
Check that all equipment is in good working order | • Sharpen knives | |
• Be sure that silos are free from leaks | ||
• In upright silos, a good distributor helps to distribute and pack silage | ||
Chop material to correct length: about 3/8 to ½ inch | • Promotes good packing and elimination of oxygen | |
• Promotes cud chewing by cow | ||
Wilt and chop during dry weather | • Prevents extensive DM losses from rain | |
• Promotes rapid drying | ||
Harvest, fill, and seal quickly | • Quick elimination of oxygen reduces DM losses from respiration and prevents growth of undesirable aerobic organisms | |
• Sealing minimizes exposure to air (tarps and sheeting) | ||
• Pack to proper density to eliminate air | ||
Allow silage to ferment for at least 21 – 28 days | • Properly ensiled forage will minimize production losses during silage changeover | |
Table 2. Amounts of common fermentation end products in various silages. | |||||
Item | Alfalfa silage, | Alfalfa silage, | Grass silage, | Corn silage, | High moisture DM corn, |
30-35% DM | 45-55% DM | 25-35% | DM 35-40% | 70-73% DM | |
pH | 4.3 - 4.5 | 4.7 - 5.0 | 4.3 - 4.7 | 3.7 - 4.2 | 4.0 - 4.5 |
Lactic acid, % | 7 - 8 | 2 - 4 | 6 - 10 | 4 - 7 | 0.5 - 2.0 |
Acetic acid, % | 2 - 3 | 0.5 - 2.0 | 1 - 3 | 1 - 3 | < 0.5 |
Propionic acid, % | < 0.5 | < 0.1 | < 0.1 | < 0.1 | < 0.1 |
Butyric acid, % | < 0.5 | 0 | <0.5 | 0 | 0 |
Ethanol, % | 0.5 - 1.0 | 0.5 | 0.5 - 1.0 | 1 - 3 | 0.2 - 2.0 |
Ammonia-N, % of crude protein | 10 – 15 | < 12 | 8 - 12 | 8 - 12 | < 10 |
Table 3. Common end products of silage fermentation. | ||
Item | Positive or negative effect | Action(s) |
pH | + | Low pH inhibits bacterial activity. |
Lactic acid | + | Inhibits bacterial activity by lowering pH. |
Acetic acid | - | Associated with undesirable fermentations. |
+ | Inhibits yeasts responsible for aerobic spoilage. | |
Butyric acid | - | Associated with protein degradation, toxin formation, and large losses of DM and energy. |
Ethanol | - | Indicator of undesirable yeast fermentation and high DM losses. |
Ammonia | - | High levels indicate excessive protein breakdown |
Acid detergent insoluble nitrogen | - | High levels indicate heat-damaged protein and low energy content. |
Table 4. Predominant fermentation pathways in silage. | |||
Type of fermentation | End products | Theoretical DM recovery (%) | Theoretical energy recovery (%) |
Homolactic (glucose) | lactic acid | 100 | 99 |
Heterolactic (glucose) | lactic acid, ethanol, CO2 | 76 | 98 |
Heterolactic (fructose) | lactic acid, acetate, mannitol, CO2 | 95 | 99 |
Yeast (glucose) | ethanol, CO2 | 51 | 99 |
Clostridia (glucose and lactate) | butyric acid, CO2 | 49 | 82 |
Table 5. Some of the more common bacteria used as silage inoculants and some reasons for their use. | |||
Organism | Type of organism | General reasons for addition | Primary end products |
Lactobacillus plantarum | Lactic acid bacteria, homolactic | rapid production of lactic acid relatively acid tolerant | Lactic acid |
Pediococcus acidilactici, cerevisiae | Lactic acid bacteria, homolacti | rapid production of lactic acid faster growing than Lactobacillus some strains show good growth at cooler temperatures some strains have good osmotolerance | Lactic acid |
Enterococcus faecium | Lactic acid bacteria, homolactic | rapid production of lactic acid faster growing than Lactobacillus | Lactic acid |
Propionibacterium shermanii, jensenii | Propionibacteria | production of antifungal compounds | Propionic and acetic acids, CO2 |
Table 6. Theoretical effect of adding a microbial inoculant containing homofermentative lactic acid bacteria on the end products of silage fermentation. | |
Item | Theoretical effect |
DM recovery | Greater recovery |
Rate of pH decline and final pH | Faster decline and lower final pH |
Ammonia nitrogen | Lower content |
Lactic acid | Greater content |
Acetic acid | Lower content |
Butyric acid | Lower content |
Ethanol | Lower content |
Fiber (NDF/ADF) | No change |
Digestibility | Increased |
Animal performance | Increased |
Table 7. A summary of animal responses to microbial inoculants between 1990 and 1995. | |||
Type of study | Intake | Gain | Milk production |
Number of studies | 6 7 | 1 5 | 3 6 |
Studies with positive responses | 28% | 53% | 47% |
Kung and Muck (1997) |
Unfortunately, there is no good way to predict the effectiveness of microbial inoculants. A model developed by Pitt (1990) suggested that inoculants would be most effective on alfalfa during cool conditions of first, third and fourth cuttings. However, there are numerous products with little or no research to support claims of improved fermentation or animal performance. Another factor which complicates the evaluating process is that the majority of bacterial inoculants are repackaged for distribution under private label and numbers of bacteria may be low and/or other additives (e.g., enzymes, fermentation extracts, minerals) are included in the formulations.
INOCULATION RATE, USE AND STORAGE
The organism(s) from microbial inoculants must be present in sufficient numbers to effectively dominate the fermentation. The most commonly recommended inoculation rate for L. plantarum-based inoculants result in a final concentration of 100,000 (or 1 × 105) colony forming units per gram of wet forage. There is limited evidence to support the suggestion of some that doubling or tripling this amount (e.g. 200,000-300,000 CFU) is more beneficial in North American silages; however higher inoculation rates are typically used on the low dry matter grass silages produced in many parts of Europe.
Most microbial inoculants are available in powder or granular form. Inoculants applied in the dry form are often mixed with calcium carbonate (limestone), dried skim milk, sucrose or other carriers.
These products can be applied by hand or by solid metering devices as per manufacturer’s recommendations. Inoculants to be applied in the liquid form come as dried powders and are mixed with water just prior to use. (Use of chlorinated water may be detrimental to the inoculant if chlorine levels exceed more than 1.5 to 2 ppm.) Application can be with a simple watering can by weighing the incoming forage load and adjusting application based on the average unloading time.
A better method is to use a metered liquid sprayer to evenly disperse the inoculant on the forage. Unused liquids should be discarded after a period of 24-48 hrs because bacterial numbers begin to decline. Microbial inoculants can be applied to the forage at a variety of locations (Table 8). However, application to forage at the chopper is highly recommended in order to maximize the time that microorganisms are in contact with fermentable substrates. Application at the chopper is more important if silage is being stored in a bunk or pile because it is difficult to achieve good distribution onto silage from a forage wagon. Distribution of the inoculant is less of a problem if it is applied at the blower of an upright silo or at the bagger. Throwing a can of dry inoculant onto a load of forage and hoping for even distribution is not an acceptable practice! Inoculants can be applied in a liquid or solid form. Data from our lab (Whiter et al., 1999) suggest that on higher DM silages (greater than about 45% DM), using a liquid based inoculant is preferable because the low moisture in these silages limits fermentation (Table 9). Inoculants applied in a liquid form may be more advantageous because the bacteria are added with their own moisture to help speed up fermentation.
Table 8. Preferred site of application for a microbial inoculant. | |||
Site of application | |||
Type of silo | First choice | Second choice | |
Bunk, pile, pit | Chopper | Silo | |
Upright bag | ------------------------------ | Chopper or Silo* | ------------------------------ |
*First choice at chopper if time between chopping and filling is more than 2 to 3 hours. |
Table 9. Preferred mode of application for a microbial inoculant. | |||
Mode of application | |||
DM content of forage | First choice | Second choice | |
< 35 to 40% | ------------------------------ | Dry or Liquid | ------------------------------ |
> 40 to 45% | Liquid | Dry | |
*First choice at chopper if time between chopping and filling is more than 2 to 3 hours. |
Storage is an important aspect of a high quality inoculant that contains live microorganisms. Some inoculants require refrigeration or freezing for optimum storage. Those that do not require cold temperatures for storage should still be kept in cool, dry areas away from direct sunlight. Moisture, oxygen and sunlight can decrease the stability of inoculants resulting in lower viable counts and a product that does not meet label guarantees. Opened bags of inoculants should be used as soon as possible and, if not completely used, probably not carried over into the next season.
MISCELLANEOUS ORGANISMS
Several microorganisms that are not homofermentative LAB have been used as silage inoculants specifically for the purpose of improving aerobic stability. For example, the Propionibacteria are able to convert lactic acid and glucose to acetic and propionic acids that are more antifungal than lactic acid. Florez-Galaraza et al. (1985) reported that addition of P. shermanii prevented the growth of molds and markedly reduced the initial population of yeast in high moisture corn where the final pH was greater than 4.5. Dawson (1994) reported similar findings in high moisture corn. Weinberg et al. (1995) saw little benefit from adding Propionibacteria to pearl millet and corn silage (final pH < 4.0) but reported improvements in the aerobic stability of wheat silage when the decline in pH was slow.
Similarly, in three studies using laboratory silos, we (Kung et al., unpublished data) did not observed beneficial effects of Propionibacteria in corn silage (final pH 3.6 to 3.8). However, Bolsen et al. (1996) reported more propionic acid, fewer yeasts and molds and greater aerobic stability in corn silage (pH of 3.6) treated with Propionibacteria. Some concerns relative to the use of Propionibacteria that have not been adequately addressed are the loss of DM (from CO2 production) and the fact that Propionibacteria have proteolytic activity. The primary reasons for the ineffectiveness of these organisms include the facts that they are strict anaerobes, they are slow growing, and they are relatively acid intolerant.
Heterolactic lactobacilli may also be useful as silage inoculants. For example, two new isolated heterolactic strains of L. plantarum have been shown to improve the aerobic stability of corn silage by an average of 28 hrs in five studies (Allman and Stern, 1999). These organisms were selected for fast growth, production of lactic and acetic acids, and the ability to suppress the growth of five major strains of yeasts that cause spoilage in corn silage.
Another heterolactic acid bacterium having potential to improve the aerobic stability of silages is Lactobacillus buchneri. Driehuis et al. (1996) reported that corn silage treated with L. buchneri was more stable than untreated silage. They suggest that improved aerobic stability was due to the ability of L. buchneri to ferment lactic acid to acetic acid and 1,2 propanediol (Oude-Elferink et al., 1999). Our laboratory (Ranjit et al., 1998) added L. buchneri to corn silage at a rate of 1 × 106 CFU/g of silage and found decreased numbers of yeasts and increased acetic acid in silage (from 1.8 to 3.6%, DMB). Aerobic stability was markedly improved by inoculation (control silage heated after 26 hrs while treated silages remained cool for more than 400 hrs in silage). Increases in acetic and propionic acids in silages treated with L. buchneri accompanied improvements in the aerobic stability of barley silage (Kung et al., 1999). We also have observed improved aerobic stability in high moisture corn treated with L. buchneri (Taylor and Kung, unpublished data, University of Delaware).
These data are exciting but some have criticized the use of heterolactic organisms because their metabolism could lead to excessive DM loss in the silo. In addition, high levels of acetic acid may depress animal intakes. To date, in research studies, losses of DM have been small and no negative effects on animal performance have been observed when feeding silages treated with L. buchneri.
Enzymes as silage additives
Enzymes were originally added to silage to partially degrade fiber to fermentable water soluble carbohydrates for use by lactic acid bacteria, which cannot use fiber as an energy source to make lactic acid. Thus, they were not designed to result in excessive degradation of fiber in the silo.
Cellulase (degrades cellulose) and hemicellulase (degrades hemicellulose) enzyme complexes are commonly mixed with microbial inoculants to form silage additives. Very few, if any, silage additives comprised only of enzymes exist on the North American market. Thus, it has been difficult to assess the effect of enzymes on silage fermentation and animal performance. In general, there have been fewer studies on silages treated with enzymes than on those treated with microbial inoculants. Effects on subsequent animal performance have also been less than found with inoculants. In a review of literature published between 1990-1995, Kung and Muck (1997) reported that the proportion of enzyme treatment studies showing positive effects on animal performance was 21% for intake (n = 29), 40% for gain (n = 10), 33% for milk production (n = 12) and 27% for feed efficiency (n = 11). The average increase in milk production was 2 lb per day in studies where milk production was enhanced. Improvements in DM digestion were only positive 9% of the time (n = 78), which leaves much speculation as to how enzyme treatment results in improved animal performance.
Buffered propionic acid-based additives
Of the short-chain fatty acids, propionic acid has the greatest antimycotic activity. It is effective in reducing yeast and molds which are responsible for aerobic deterioration in silages. The antimycotic effect of propionic acid is enhanced as pH declines, making it an ideal candidate for improving the aerobic stability of corn silage where pH is low. In the past, aerobic stability was improved when large amounts of propionic acid (1 to 2% of the DM) were added to silage, but the high percentage of acid restricted fermentation in these cases. Propionic acid is also difficult to handle because it is corrosive.
Thus, the acid salts, e.g., calcium, sodium and ammonium propionate, have become more widely used in commercial products. The efficacy of propionic acid and its salts is closely related to their solubility in water. The stronger the bond is between the acid and base, the less soluble the product is and thereby less effective in inhibiting fungi. Among these salts, ammonium propionate is most soluble in water (90%), followed by sodium propionate (25%) and calcium propionate (5%). Most current additives containing buffered propionic acid and other antifungal components (e.g., citric acid, benzoic acid, and sorbic acid) have low suggested rates of application (2-4 lb per ton of fresh forage weight). These low application rates usually do not affect silage fermentation, but reduce the numbers of yeasts that cause aerobic spoilage and improve aerobic stability (Table 10). Several additives containing buffered propionic acid are available that were designed for use in the total mixed ration just prior to feeding to prevent heating and spoiling in the feed bunk. However, research from our lab suggests that controlling yeasts at the time of ensiling is more efficient than trying to control their numbers and metabolism in the feed bunk.
Table 10. Effect of a propionic acid-based additive on the number of yeasts and hours of aerobic stability of corn silage. | ||
Treatment*, application rate | Yeast in silage (Number/g) | Aerobic stability (hrs)** |
Untreated | 257,000 | 65 |
Product A, 2 lb/ton of wet silage | 27,000 | 120 |
Product A, 4 lb/ton of wet silage | 2,800 | >160 |
*Product A contained buffered propionic acid (primary active ingredient) and other active ingredients. **Hours before the temperature of the silage rose more than 20C. Kung et al., 1998. |
Conclusions |
Silage additives can be useful tools to improve silage quality and animal performance; however, they are not replacements for good management practices. The question of which additive to use can sometimes be a difficult one. Table 11 shows some suggestions for use of silage additives. Cost of the product should not be the most important factor when choosing an additive! Proof of efficacy and cost should be considered together. Why buy a cheap additive that is ineffective? In contrast, the most expensive additive might not be the best, either. How should one evaluate a silage additive? In my opinion, in choosing an additive in North America one should require an extensive database proving efficiency in a broad range of conditions (crop, moisture content, etc) in three areas: 1) improvements in animal production; 2) imrovements in DM or nutrient recovery; 3) improved aerobic stability. Finally, choose an additive from a reputable company that stands behind their products and offers excellent technical service support. |
Table 11. Some suggestions for use of silage additives. | |
Major observation | Additive of choice |
1. Consistently make good quality silage. | 1. Homolactic acid based inoculant. |
2. No significant heating problems. | |
1. Consistently make good quality silage. | 1. Homolactic acid based inoculant and |
2. Some heating problems during warm weather. | 2. Buffered propionic acid preservative added to TMR at feeding. |
1. Usually make good quality silage. | 1. Homolactic acid based inoculant and |
2. Spoiled or hot silage usually only at silo opening and when feeding out the last silage from silo. | 2. Buffered propionic acid preservative or inoculants designed to improve aerobic stability on several first and last loads into silo. |
1. Consistently have problems with heating silage. | 1. Buffered propionic acid preservative at ensiling or |
2. Inadequate daily removal of silage leading to hot feed. | 2. Microbial inoculants designed to improve aerobic stability. |
1. For bunk, pit, or drive-over silos, significant spoilage on top layer even after covering. | 1. Buffered propionic acid preservative or |
2. Microbial inoculants designed to improve aerobic stability only on last loads into silo. | |
1. Extremely dry forage or forage chopped too long | 1. Buffered propionic acid preservative or |
2. Microbial inoculants designed to improve aerobic stability. |
References
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