Types of Elisa

Amine Mernizi
Dear alle,
If you use plates coated with antigen It depends alle about the purify of the antigen this van be native purified virus OR recombinant. Native purified inactivated virus might still haber cell antigenen which can Puck up antibodies finally inducing false positives. Otherside if you use recombinant antigens you can mis positive since glycolysation might be different OR nog present at all.
Blocking testing might become more specific and one van use leds purified antigen. Some test like for influenza pick up antigens An exsample of this is influenza NP which can be captured by using monoclonal antibodies and annenzhme labelled conjugatie same type of assay is used for detecting ALV p27 antigen.
If you want to read more about these assays you can find more information on our website www.evlonline.org.
Don't hesitate to ask me if you need more info OR need help with development of assays.
Best regards
Rob.