Discussion created on 07/22/2011

Recombinant Poultry Vaccines

Forum: Recombinant Vaccines

Dear members,

I would like to know your opinion about the use of recombinant vaccines to prevent ILT, Newcastle disease and Marek´s Disease in chickens. What are the advantages and disadvantages in comparison with Conventional Poultry Vaccines?

Look forward to hearing your experiences!

Analía Göttig
Community Manager
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Maqsood Jaffery Maqsood Jaffery
Doctor of Veterinary Medicine
July 27, 2011

Future disease control through vaccination will mainly depend upon recombinant vaccines. It is in use at present in sizable proportion.

For use of the recombinant vaccines it is mandatory/ compulsory for vaccine to be used to be registered in country of use after fulfilling the legal requirements. First you have to look please weather theses vaccines are allowed legally in the country of use.

Recombinant vaccine is the induction of antigenic protein producing/coding genome into some other vaccine, so that by vaccinating one vaccine, you also get the immunity against the other disease whose genome has been inducted in to vector vaccine. For examle Nd vaccine as recombinant with HVT vaccine , IBD with HVT vaccine and so on.

Following are the some of the advantages of recombinent vaccine.

A: - By administration of one vaccine (as recombinant vaccines are live vaccines), recombined/ inserted genome also replicates along with virus/antigen being used as vector. Immunity is produced against both by single administration.

B: - It has least disturbance against maternal antibodies which interfere with immunity process. This vaccine can be done in ovo i-e in incubating egg, so when the chick is hatched it will have immunity levels against the diseases for which vaccine has been done. In this way it will give better protection in areas of high challange for pathogens/ diseases.
Recombinant vaccine either not repeated or with long intervals depending upon the health /titer of the bird against specvific vaccine used.
This vaccine can be administrated at hatchery by skilled staff or at farm to get better protection.While if not recombinant vaccine used then vaccine used at farm and administration of vaccine not reliable, while at hatchery or in ovo it is being done by automated machines by skilled staff and produce better results.

C: - Disadvantages. as it has not long usage record so disadvantage should be highlighted after use of recombinant vaccine for some time.

D: - By advancement in molecular techniques recombinant vaacine making trend will increase.

E: - It does not disturb maternal antibodies. As in IBD single IBD dose given through injection at day 1 will not interfere with maternal antibodies and will give protection for long time with single dose administration, which avoids stress of vaccination at least against for revaccinations. This will help in better performance.

Regards,
Syed maqsood Haider Jaffery

Reply
Dr Jaydip Mulik Dr Jaydip Mulik
Master Of Veterinary Science & Master of Business Administration
August 2, 2011
Thanks Dr Maqsood Jaffery ,

You have stated the Pros & Cons of recombinant vaccines with deep thoughts.

regards,

Dr Jaydip
Reply
August 2, 2011

In addition to the live vector vaccines based on different viruses (mainly on HVT), inactivated vaccines for poultry has also hugh advantages in its safety and efficacy. ABIC biological laboratories (Phibro animal health care corporation) develop and produces several recombinant vaccine against infectious bursal disease and against egg drop syndrom disease. These vaccines are very efficient and more targeted to produce neutralizing antibodies against the specific pathogen. As I know Dow agroscience developed recently subunit vaccine against NDV based on HN protein, I wonder if somebody have idea for its efficacy?

Best regards,

Dr. Avi Zrachya

ABIC biological laboratories Ltd.

Reply
Dr Jaydip Mulik Dr Jaydip Mulik
Master Of Veterinary Science & Master of Business Administration
August 3, 2011
Dear Dr. Avi Zrachya,

I feel you have the expertise in the field of Immunology & working in the same environment so i wish to know

more about the Pros & Cos of Live vaccines, Killed Vaccines , and recombinant vaccines.

Also i will provide here some MAB values tested for DOC broilers for different Diseases on which i wish to

get better guidance to frame the best vaccination schedule.( Especially on IBD as MAB differs too much)
MAB titers (for Day Old chicks):
1)

Prashant Bhor Sample received on 03.06.2011

Age-DOC
ND HI IB Titers IB Units IBD Titers IBD Units ILT Titers ILT Units
6 6675 58.2 23086 111.8 1118 25.7
6 7137 60.7 24821 118.2 1794 33.0
7 10559 77.6 35242 154.6 3160 44.4
7 18844 111.4 50850 204.8 4362 52.5
7 24890 132.6 65288 248.0 5130 57.2
8 26399 137.6 71423 265.6 5441 59.0
9 32762 157.5 83622 299.7 7154 68.1

Mean 7.1 18180.9 105.1 50618.9 200.4 4022.7 48.6
Median 7.0 18844.0 111.4 50850.0 204.8 4362.0 52.5
CV 53% 44% 49%

(Age-1days, Broiler, Flock Size. 34680).
ND HI Log2 titers: 7x2, 8x6 Mean=7.8 Median=8.0
IB ELISA titers: 7504, 8677, 12325, 13813, 16169, 19991, 23443
Mean=14560 Median=13813 CV=37%
IB ELISA Units: 62.6, 68.6, 85.4, 91.8, 101.3, 115.6, 127.8
Mean=93.3 Median=91.8
IBD ELISA titers: 3975, 5554, 6535, 9400, 10255, 11421, 11463, 15311
Mean=9239 Median=9827 CV=40%
IBD ELISA Units: 29.1, 37.5, 42.5, 56.2, 60.1, 65.2, 65.4, 81.6
Mean=54.7 Median=58.2

Samples dated 31/03/2010, received on 05/04/2010
Titer results: Doc, Broiler
ND HI Log2 titers: 5x3, 6x5, 7x5, 8x2 Mean= 6.4 Median= 6.0
IBD ELISA titers: 6282, 6548, 6914, 6943, 7069, 7178, 5648, 9552, 9904, 10337, 12424,
13384, 15308, 1713.6, 19102 Mean=10448 Median= 9552 CV=39%
IBD ELISA Units: 40.8, 41.3, 42.6, 44.5, 45.2, 45.7, 52.7, 56.9, 58.5, 60.4, 69.6, 73.6,
81.6, 89.0, 96.7 Mean= 59.9 Median=56.9

Please find MAB titers of samples dated 10-02-2011 for M/S Shobha Bhalsingh PF,
Samples dated 10/02/2011, received on 15/02/2011
Titer results:
DOH 09-02-2011, Total Chicks-4668
ND HI Log2 titers: 7x4, 8x5, 9x1 Mean=7.7 Median=8.0
IBD ELISA titers: 9690, 11130, 14651, 14673, 14738, 18532, 19836, 21670, 23119, 24039
Mean=17207 Median=16635 CV=27%
IBD ELISA Units: 57.5, 63.9, 78.9, 79.0, 79.3, 94.5, 99.6, 106.5, 111.9, 115.3
Mean= 88.6 Median=86.9


Awaiting your feed back on the same> From the same source of chicks why this much deviations in ttre reports.


Regards,

Dr. Jaydip


Reply
August 3, 2011

Dear Dr. Jaydip,

Many thanks for your kind response,

In the following you could find all vaccine types related on two opposite parameters: Safety vs. Efficacy.

EFFICACY --- nucleic acid vaccines - subunit - virus like particles - inactivated virus - non-replicating viral vectors - replicating vectors - jennerian - live attenuated virus --- SAFETY

It is obvious that replicating vaccines will enhance the immune system better than non-replicating vaccines. That is due to the activation of the two arms of the immune system. On the other hand, subunit vaccines is more safe to use and from our experience it is also very efficient!
For example: IBDV encode two proteins which creat its envelop and coating the viral genome - VP2 and VP3. It was shown that VP2 only is important for inducing neutralizing antibodies against the virus. For this all the recombinant vaccines against gumboro involve this protein either for live vector vaccines or subunit vaccines.

For vaccination workplan against IBDV for broilers you could contact our veterinars Dr. Beny perelman (beny.perelman@pahc.com) or Dr. Udi Ashash (udi.ashash@pahc.com).

Hope that this information helps,

BR,

Avi.

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