Characterization of a Mutant Buttiauxella Phytase using Phytic Acid and Phytic Acid-Protein Complex as Substrates
Published on: 09/01/2014
Author/s : Shukun Yu1,3*, Marie Fjordside Kvidtgaard1,2, Mai Faurschou Isaksen1, and Soren Dalsgaard1 (1 Enzyme R&D, DuPont Industrial Biosciences, Aarhus, Denmark; 2 Department of Food Sciences, Aarhus University, Tjele, Denmark ;3 Department of Biotechnology, Lund University, Lund, Sweden)
Phytase is the enzyme that hydrolyzes phytic acid and its salt phytate (myo-inositol hexakisphosphate, IP6), resulting in the formation of inositol pentakis-, tetrakis-, tris-, bis- and monophosphate (IP5-IP1) in a stepwise manner and five inorganic phosphates (Pi) (Skoglund et al., 1998; Greiner et al., 2000). Inclusion of phytase in animal feed not only makes the phytate-bound p...