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Symposium on Gut Health in Production of Food Animals 2022
The following technical article is related to the event::
Symposium on Gut Health in Production of Food Animals 2022

Nutrient transporters and tight junction expression and cecal short-chain fatty acid profile in Eimeria-challenged broilers fed diets with different levels of xylo-oligosaccharides

Published on: 7/22/2022
Author/s : Y. Lin and O. Olukosi / University of Georgia, Athens, GA, USA.
Summary

A total of 252 Cobb 500 male broiler chicks were used in a 21-d experiment to study the possibility of xylo-oligosaccharides (XOS) helping to recover gut impairment in Eimeria-challenged broilers by regulating the expression of nutrient transporters and tight junctions, and cecal short-chain fatty acids (SCFA), which is an indicator of bacterial status. Birds were allocated to 6 treatments in a 3 × 2 factorial arrangement (3 corn-soybean diets with 0, 0.5, 1 g/kg XOS × with or without Eimeria challenge). Each treatment had 6 replicates with 7 birds per replicate. Challenged groups were inoculated with a solution containing E. maxima, E. tenella, and E. acervulina oocysts on d 15. On d 21, jejunal tissue was collected for gene expression analysis and cecal content was collected for SCFA analysis. The Eimeria × XOS interaction for tight junction claudin 1 showed that both 0.5 and 1 g/kg XOS alleviated (P < 0.05) Eimeria-induced claudin 1 upregulation. The Eimeria × XOS interaction for sugar transporters showed the extent of Eimeria-induced GLUT2 and GLUT5 downregulation was smallest in the 0.5 g/kg XOS supplemental treatment. In addition, Eimeria upregulated (P < 0.01) tight junction JAM2 and glucose transporter GLUT1 but downregulated (P < 0.01) the peptide transporter PepT1, amino acid transporters rBAT, CAT2, y+LAT2, and zinc transporter ZnT1. Eimeria decreased (P < 0.05) cecal saccharolytic SCFA acetate, butyrate and total SCFA, but increased (P < 0.05) cecal branched-chain fatty acids isobutyrate and isovalerate. The supplementation of XOS tended to decrease the concentration of isobutyrate (P = 0.080) and isovalerate (P= 0.062). In conclusion, Eimeria challenge triggered changes in expression of tight junction and nutrient transporter genes. Supplemental XOS helped reverse the gene expression changes in tight junction claudin 1 and glucose transporter GLUT2 and GLUT5, and showed the potential of alleviating the Eimeria-induced unfavorable cecal fermentation pattern.

Key Words: xylo-oligosaccharides, Eimeria, tight junction, nutrient transporter, short-chain fatty acids (SCFA).

        

Presented at the 9th Symposium on Gut Health in Production of Food Animals, St. Louis, USA, 2021. For information on the next edition, click here.

 
Author/s :
Dr. Oluyinka A. Olukosi is the Senior Poultry Research Scientist at the Avian Science Research Centre of the SRUC (formerly SAC) since 2010 where he is leading the poultry nutrition research activities. He obtained his PhD degree in Animal Sciences from Purdue University in 2008 followed by a two-year post-doctoral training at the same university. His research is on feedstuff evaluation, the utilisation of feed additives in animal nutrition, whole-body nutrient utilisation and nutrient partitioning. He also works in areas of nutrition and health interaction.
 
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