- Earlier, it was hypothesized that growth promoting effects of Antimicrobial Growth Promoters (AGPs) in poultry feed are attributed to their non-antibiotic anti-inflammatory properties.
- Inflammation causes a reduction in growth (through inappetence and muscle catabolism), birds without any inflammation can reach 100 % of their genetic growth potential.
- Among the proposed anti-inflammatory alternatives is Lumance® (a commercial blend of SCFA, MCFA, Essential oil and Plant extract) with anti-inflammatory activities (Innovad NV/SA, Belgium).
- Recently the use of acute phase proteins such as C-reactive protein (CRP), as a nonspecific clinical markers of health problems and inflammation has received considerable attention.
- This study aimed to confirm the anti-inflammatory properties of (Lumance®) in an in-vitro model of inflammation and correlate it with in-vivo growth promotion in laying hens using plasma CRP as a potential marker of inflammation.
Materials and Methods
- Anti-inflammatory activity of Lumance® was tested using the RAW 264.7 assay, as described by Wu et al (2003).
- Sixty Hisex Brown laying hens at the age of 30 weeks were divided into five dietary treatments (Lumance® at 0, 0.5, 1, 1.5 and 2 Kg/ton) with three replicates of four birds each.
- At weeks 38, three laying hens from each treatment were euthanized and plasma level of C-reactive protein (CRP) was determined with a commercial ELISA kit
We have reported previously the beneficial effects of Lumance® on production and egg quality of these laying hens (Kliseviciute, et al, 2013).
- Change in the concentration of plasma CRP, is one of the major hallmarks of inflammation, which are associated with growth performance and several economically important parameters.
- These results suggest that Lumance® can reduce the inflammation and consequently can improve the production and egg quality of laying hens.
- A combination of different ingredients with anti-inflammatory effect hold the promising approach to replace AGPs.