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The following technical article is related to the event:
22nd European Symposium on Poultry Nutrition - ESPN 2019

A low level of C-reactive protein (CRP) as a potential marker for lower inflammation in laying hens

Published on: 9/4/2019
Author/s : Alireza Khadem ¹,², Markella Al-Saifi², Milena Sevastiyanova² and Christos Gougoulias ². 1Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Belgium. ² Innovad, Belgium. Corresponding author: Alireza Khadem.


  • Earlier, it was hypothesized that growth promoting effects of Antimicrobial Growth Promoters (AGPs) in poultry feed are attributed to their non-antibiotic anti-inflammatory properties.
  • Inflammation causes a reduction in growth (through inappetence and muscle catabolism), birds without any inflammation can reach 100 % of their genetic growth potential.
  • Among the proposed anti-inflammatory alternatives is Lumance® (a commercial blend of SCFA, MCFA, Essential oil and Plant extract) with anti-inflammatory activities (Innovad NV/SA, Belgium).
  • Recently the use of acute phase proteins such as C-reactive protein (CRP), as a nonspecific clinical markers of health problems and inflammation has received considerable attention.
  • This study aimed to confirm the anti-inflammatory properties of (Lumance®) in an in-vitro model of inflammation and correlate it with in-vivo growth promotion in laying hens using plasma CRP as a potential marker of inflammation.

Materials and Methods 


  • Anti-inflammatory activity of Lumance® was tested using the RAW 264.7 assay, as described by Wu et al (2003).
  • Sixty Hisex Brown laying hens at the age of 30 weeks were divided into five dietary treatments (Lumance® at 0, 0.5, 1, 1.5 and 2 Kg/ton) with three replicates of four birds each.
  • At weeks 38, three laying hens from each treatment were euthanized and plasma level of C-reactive protein (CRP) was determined with a commercial ELISA kit


We have reported previously the beneficial effects of Lumance® on production and egg quality of these laying hens (Kliseviciute, et al, 2013).


  • Change in the concentration of plasma CRP, is one of the major hallmarks of inflammation, which are associated with growth performance and several economically important parameters.
  • These results suggest that Lumance® can reduce the inflammation and consequently can improve the production and egg quality of laying hens.
  • A combination of different ingredients with anti-inflammatory effect hold the promising approach to replace AGPs.

Bibliographic references

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