Engormix/Poultry Industry/Technical articles
XXII Congresso Latino-Americano de Avicultura 2011
The following technical article is related to the event::
XXII Congresso Latino-Americano de Avicultura 2011

Alternatives to growth promoters

Strategies for finding alternatives to growth promoters

Published on: 10/20/2011
Author/s : Peter Ferket (Department of Poultry Science, College of Agriculture and Life Sciences, North Carolina State University)
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Summary

Gut health may be of greatest concern among poultry producers because of its impact on economic sustainability, and their customers’ concern about food safety and traceability. Gut health and nutrition are intricately dependent upon one another and it is often difficult to distinguish which one is the predisposing factor. To resolve gut health problems, one must first be able to identify the nature of a gut health problem and understand the fundamentals and modifiers of enteric development. A healthy gut is one that has a stable and diverse microbial ecosystem. Indeed, diet formulation, feed additives, and feed form must be considered regarding how they affect the colonization of enteric pathogens. Structural properties of the feed that stimulate gizzard motility has been demonstrated to promote reverse peristalsis, thereby improving the foregut digestion of proteins, fat, and starches leaving little for competitive microbiota to thrive. In contrast, viscous non-starch polysaccharides that impede reverse peristalsis and digestion of protein, fat, and starches in the foregut of poultry will cause the competitive microbiota (pathogens) to thrive. Strategic use of different feed additives can be used to stabilize the enteric ecosystem. These enteric conditioning feed additives include antibiotics, probiotics, prebiotic non-starch polysaccharides, essential oils, organic acids and short-chain fatty acids, and mananoligosaccharide derivatives of yeast cell wall, and microbial enzymes. Understanding the mode of action of these feed additives is necessary to design compatible feed additive programs to control gut health.

Introduction
Gut health has a great influence on the growth performance and welfare of poultry, as it affects feed digestion, nutrient absorption, protein and energy utilization, immunity and disease resistance, metabolism, and physiology.  Indeed, gut health may be of greatest concern among poultry producers because of its impact on economic sustainability, and their customers´ concern about food safety and traceability.  Gut health and nutrition are intricately dependent upon one another.  Optimum dietary nutrient utilization cannot be realized unless the gut is in a healthy state.
Traditionally, intestinal health has been largely dependent on prophylactic and therapeutic uses of antibiotics. However, the voluntary or legislated limits on the use of antibacterial feed additives for poultry are requiring changes in the methods to maintain good intestinal health. Today, the focus needs to be on earlier establishment of immunity and intestinal integrity if birds are to reach their maximum potential for growth and feed efficiency. In modern broilers, attention to the first weeks of life is critical to achieve the best possible performance later. High quality nutrients provided early in life are needed to ensure the development of immunity, microbial intestinal maturity and proper tissue building in the intestinal tract. Therefore, understanding the role of feed formulation to optimize gut development and health is vital for achieving future sustainability and for improving the efficiency and environmental acceptability of poultry production. The objective of this paper is to review nutritional strategies to optimize gut development and health and control pathogen colonization with emphasis on potential natural alternatives to antibiotics.
 The Microbial Ecosystem of the Avian Gut
Finding alternatives to antibiotics to control gut health can be more easily understood if one considers it from an ecological perspective.  It has much to do with maintaining a stable ecosystem in the hindgut for symbiotic bacteria to flourish rather than allow conditions that favor the proliferation of pathogenic or competitive microbes.   Birds can harbor an extensive and diverse microflora throughout the digestive tract, but they are mainly hind-gut fermenters, accommodating large numbers of different microbes in the ceca. The microorganisms in the intestinal tract become attached to mucosal surfaces or food particles, or they remain free-living in the lumen. Micro-communities in different parts of the tract develop only after the successive establishment of some types of organisms and a decline of others, which characterize symbioses (Hentschel et al., 2000). In poultry, bacterial populations resembling those of adult small intestine are present within two weeks of hatching, but it takes 30 or more days in the ceca to develop a stable and dynamic population (Barnes et al., 1972). The slow rate of development appears to be due to the highly sanitized hatching and rearing conditions, the lack of contact with the mother hen, and the use of antimicrobial feed additives commonly used in commercial poultry production.
Good gut motility is necessary for proper food digestion, nutrient absorption, and maintaining a healthy gut ecosysem.  Textural properties of feed (fiber content, particle size, and particle integrity) are important for proper gizzard musculature and motility.  The gizzard is the "pace-maker" of normal gut motility in birds (Duke, 1994).  Unlike mammals, vigorous gut refluxes (reverse peristalsis) are normal in birds as an adaptation to compensate for a short intestine. The refluxes serve to re-expose intestinal digesta to gastric secretions, vigorously mix digesta with enzymes to enhance digestion, enhance nutrient absorption over a short segment of the gut, and discourage microbial proliferation that may cause disease or compete for nutrients.  Enteric disorders, such as diarrhea, swollen proventriculus, and gizzard erosionmay be partially a consequence of dysfunctional gut motility associated with processed feed characteristics. The primary objective of modern feed manufacturing (grinding, post-mix grinding, steam conditioning, expansion, and pelletizing) is to reduce the bird´´´´s "work" of feed prehension and enhance digestion for the sake of maximizing feed conversion efficiency.  Although all this mechanical work invested into processing feed reduces the work load of the gizzard to grind the ingested food, it also leads to atrophy and malfunction of the gizzard and associated gut motility (Cumming, 1994).  Poor peptic digestion by pepsin in the gizzard will result in less efficient peptic digestion by trypsin and chymotrypsin in the duodenum.  Consequently, more undigested proteins end up in the hindgut where they are subject to microbial fermentation.  Poor protein digestibility will cause an undesirable shift in the hindgut microflora towards proteolytic and pathogenic bacteria.
Dietary Effects on Intestinal Microflora
Several strategies have been proposed as a means to manage intestinal microflora and gut health through diet formulation. Growth-promoting antibiotics (AGP) work in part by decreasing the microbial load in the intestinal tract, resulting in a reduction in energy and protein required to maintain and nourish the intestinal tissues; thus, more nutrients are partitioning toward growth and production. In contrast, most "natural" feed additives do not reduce overall microbial loads, but they alter the intestinal microflora profile by limiting the colonization of unfavorable bacteria and promote the activity or growth of more favorable species. AGP alternatives modulate gut health by several possible mechanisms: altering intestinal pH; maintaining protective intestinal mucins; selection for beneficial intestinal organisms or against pathogens; enhancing the fermentation volatile short-chain fatty acids; enhancing nutrient uptake; and increasing the humeral immune response (Ferket, 2003). Although there is growing scientific support for many of these antibiotic replacements (Yang et al., 2009), the claim of efficacy is in many cases inadequately substantiated (Rosen, 2003). The search has been for a single intervention or product to replace antibiotics, and this has shown to be less efficient than a multi-factorial approach (Collett, 2004). A number of options are available for enhancing the performance of poultry in the absence of specific feed-additive antibiotics. However, an alternative strategy or program must yield comparable economic return, and production efficiency must be sustainable if it is to be accepted for commercial use.
Probiotics: A probiotic (direct-fed microbial) is defined as "a live microbial feed supplement that beneficially affects the host animal by improving its intestinal microbial balance" (Fuller, 1989).  Lactobacillus and Bifidobacterium species have been used most extensively in humans, whereas species of Bacillus, Enterococcus, and Saccharomyces yeast have been the most common organisms used in livestock (Salminen et al., 1998). These symbiotic microorganisms competitively exclude (Nurmi and Rantala, 1973) pathogenic microorganisms by the following possible mechanisms: 1) lowering the pH through production of fermentation acids; 2) competing for mucosal attachment and available nutrients; 3) producing bacteriocins; 4) stimulating the gut associated immune system through cell wall components (Nousiainen and Setala, 1998); and 5) increasing the production of short-chain fatty acids, which have bacteriostatic and bactericidal properties (Fuller, 1977) and stimulate intraepithelial lymphocytes, and natural killer cells (Ishizuka and Tanaka, 2002; Ishizuka et al., 2004).
Probiotics have some disadvantages in comparison to other modulators of enteric microflora (Fooks et al., 1999; Isolauri et al., 2004). Relatively few species of microorganisms can be considered for use in probiotics products due to their limited knowledge of culturability and required conditions for application and storage, such as extreme anaerobiosis. Probiotics have a short shelf-life and most are labile to excessive heat and pressure during feed processing. Some probiotic microorganisms may be reduced or eliminated by the low pH in the gizzard, and thus have little effect in the lower intestinal tract where pathogens pose problems. If a probiotic is added to the drinking water, the chlorine sanitizer may adversely affect its survivability. Acidification would be a better sanitizer than chlorine when delivering a probiotic via the drinking water.
Herbs, spices and essential oils have been used to make human foods more appetizing for centuries, and many of them are recognized for their health benefits. Essential oils have long been recognized for their anti-microbial activity (Lee et al., 2004a), and they have gained much attention for their potential as alternatives to antibiotics. Lee and Ahn (1998) found that cinnamaldehyde, derived from the cinnamon essential oil, strongly inhibit Clostridium perfringens and Bacteroides fragilis in vitro, and moderately inhibit Bifidobacterium longum and Lactobacillus acidophilus isolated from human. Also, a wide range of in-vitro anti-microbial activities of essential oils derived from cinnamon, thyme and oregano have been published (Deans and Ritchie, 1987; Lee et al., 2004a). Although the exact anti-microbial mechanism of essential oils is poorly understood, it may be associated with their lipophilic property and chemical structure (Lee et al., 2004b).
Helander et al. (1998) investigated how two isomeric phenols, carvacrol and thymol, and the phenylpropanoid, cinnamaldehyde, exert their antibacterial effects on E. coli O157 and S. Typhimurium. Both carvacrol and thymol disintegrated the membrane of bacteria, leading to the release of membrane-associated materials from the cells to the external medium. Conversely, cinnamaldehyde exhibited its antibacterial activity due to its lipophilicity of terpenoids and phenylpropanoids, which can penetrate the membrane and reach the inner part of the cell and impair bacterial enzyme systems. Therefore, these plant-based phenolic compounds have antimicrobial effects similar to antibiotic compounds produced by fungi. As with antibiotics, continued use of these plant-based antimicrobials may result in the development of resistance in some pathogenic bacteria (Lee et al., 2004a). However, more research is necessary to confirm this risk. To be as effective as growth promoters, these herbal antimicrobial compounds must be supplemented to the feed in a more concentrated form than found in their natural state, which will increase usage costs.
Acidifiers and organic acids have been used for decades in feed preservation, protecting feed from microbial and fungal destruction or to increase the preservation effect of fermented feeds (e.g. silages). Because organic acids have strong bacteriostatic effects, they have been used as Salmonella-control agents in feed and water supplies for livestock and poultry (Ricke, 2003). The most common organic acids in animal nutrition are citric acid, propionic acid, fumaric acid, lactic acid, formic acid and benzoic acid. Additionally, some other available acidifiers and organic acids have been shown to have some antimicrobial activity (Russell, 1992). The antibacterial activity of organic acids is related to the reduction of pH, as well as their ability to dissociate and easily enter the microbial cell by both passive and carrier-mediated transport mechanisms. Once in the cell, the organic acid releases the proton H+ in the more alkaline environment, resulting in a decrease of intracellular pH. This hinders microbial metabolism by inhibiting the action of important microbial enzymes and forces the bacterial cell to use energy to export the excess of protons H+, ultimately resulting death by starvation. In the same matter, the protons H+ can denature bacterial acid sensitive proteins and DNA. Generally lactic acid bacteria are able to grow at relatively low pH, which means that they are more resistant to organic acids than more pathogenic species. Lactic acid bacteria, like other Gram-positive bacteria, have a high intracellular potassium concentration, which counteracts acid anions (Russell and Diez-Gonzalez, 1998). The use of organic acids has not gained as much attention in poultry production, partly because limited positive responses in weight gain and FCR (Langhout, 2000). However, Vogt et al. (1982) reported a positive influence on either FCR or growth performance by dietary supplementation of fumaric acid, propionic acid, sorbic acid and tartaric acid in broiler diets. Dietary supplementation of coated sodium butyrate was also found enhance growth performance of broilers, attributed to better mucosal development (Malheiros and Ferket, 2010). 
Prebiotics are dietary components that are not digested by the host, but they benefit the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the gut, predominantly those that produce short-chain fatty acids. Prebiotics have several advantages over probiotics where culture viability needs to be maintained. Prebiotics has been shown to stimulate enteric colonization of unculturable bacteria (Rastall et al., 2005; Konstantinov et al., 2003) that discourage the colonization of enteric pathogens, and they have the advantage of being more stable to the heat and pressure incurred during feed processing. Any feed ingredient that enters the large intestine is a potential prebiotic, but it must be fermented by microorganisms that benefit the host to be effective (Lan, 2004). Most current attention and successes have been derived using non-digestible oligosaccharides, especially those that contain fructose, xylose, galactose, glucose and mannose (Gibson, 1998). Oligosaccharides and polysaccharides are preferentially utilizable by Bifidobacteria (Yazawa et al., 1978) and other lactic acid-producing bacteria that modulate gut associated immune function (Swanson, 2002; Manning and Gibson, 2004). The dominant prebiotics are fructooligosaccharide products (FOS, oligofructose, and inulin). However, trans-galactooligosaccharides, glucooligosaccharides, glycooligosaccharides, lactulose, lactitol, maltooligosaccharides, xylo-oligosaccharides, stachyose, raffinose, and sucrose thermal oligosaccharides have also been investigated (Monsan and Paul, 1995; Orban et al., 1997; Patterson et al., 1997; Piva, 1998; Collins and Gibson, 1999).
Some structural carbohydrate components of NSP have been used for many years in poultry diets as fiber of one kind or another, and they have been studied as potential prebiotics. Rafinose (galactooligosaccharides) can modify the colonic microflora by lowering some Gram-negative bacteria, such as coliforms, and increasing potentially health-promoting bacteria, such as bifidobacteria and Lactobacillus (Matteuzzi et al., 2004). Galactomannan from partially hydrolyzed guar gum has been reported to reduce diarrhea (Takahashi et al., 1993) and improve intestinal microflora (Okubo et al., 1994). Galactomannans also can suppress the colonization of Salmonella Typhimurium in vitro (Oyofo et al., 1989) and Salmonella enteritidis in laying hens (Ishihara et al., 2000). Besides its effect on microbial fermentation, arabinoxylan has also been shown to activate a macrophage cell line in the broiler intestine and thus decrease the enteric pathogen colonization (Zhang et al., 2004).
Mannan oligosaccharide (MOS) is derived from mannans on yeast cell surfaces and is not used as a substrate in microbial fermentation; but, it still exerts significant growth-promoting effect by enhancing the animal´´´´s resistance to enteric pathogens. Based on the literature, MOS enhances an animal´´´´s resistance to enteric disease and promotes growth by the following means: 1) inhibits colonization of enteric pathogens by blocking bacterial adhesion to gut lining; 2) enhances immunity; 3) modifies microflora fermentation to favor nutrient availability for the host; 4) enhances the brush border mucin barrier; and 5) reduces enterocyte turnover rate. MOS act as high affinity ligands, offering a competitive binding site for a certain class of bacteria (Ofek et al., 1977). Gram-negative pathogens with the mannose-specific Type-1 fimbrae attach to the MOS instead of attaching to intestinal epithelial cells and they move through the gut without colonization. Spring et al. (2000) observed five of seven strains of E. coli and 7 of 10 strains of Salmonella typhimurium and S. enteritidis agglutinated MOS and Sac. cerevisiae cells. However, strains of S. choleraecuis, S. pullorum, and Campylobacter did not lead to agglutination. Although MOS does not bind clostridia, it does reduce clostridia numbers in some trials, possibly by enhancing the mucin barrier or stimulating gut-associated immunity.
MOS has been shown to have a positive influence on humoral immunity and immunoglobulin status. Good immune response is a metabolically more efficient means to resist disease than an active inflammatory response (Humphrey et al., 2000). Savage et al. (1996) reported an increase in plasma IgG and bile IgA in poults fed diets supplemented with MOS. An increase in antibody response to MOS is expected because of the ability of the immune system to react to foreign antigenic material of microbial origin. Portions of the cell wall structure and MOS of the Saccharomyces organism has been shown to highly antigenic (Ballou, 1970). MOS can enhance humoral immunity against specific pathogens by preventing the colonization leading to disease, yet allowing them to be presented to immune cells as attenuated antigens. Indeed as MOS facilitates the secretion of IgA into the gut mucosa layer, pathogenic agents become more labile to the phagocytic action of gut-associated lymphocytes.
All animals reared under commercial field conditions are subjected to immunological stress depending on the pathogen load in their environment and the vaccination program. The release in cytokines associated with inflammation and the innate immune response results in fever (which reduces appetite), causes the mobilization of body reserves (glucose, aminoacids, and minerals) away from liver, muscle and bone, suppresses nutrient absorption in the gut, and increases body fluid losses as diuresis and diarrhea. The positive growth-performance effects observed among animals fed MOS may be partly due to its effect on acute immunological stress. Although MOS may enhance humoral immunity, there is some evidence that it may suppress the pro-inflammatory immune response that is detrimental to growth and production (Ferket, 2002). Under commercial conditions where birds are subjected to chronic immunological stress, MOS may help reduce the pro-inflammatory response and associated depression in feed intake and growth. The beneficial effects of dietary MOS on the gut microflora, nutrient utilization, and growth performance may also be associated with changes in brush border morphology and how it influences enteric disease resistance. Ferket (2002) reported dietary supplementation of MOS had a significant effect on intestinal villi morphology of turkey poults in comparison to those fed non-medicated control or virginiamycin-supplemented diets. Dietary MOS supplementation significantly increased villi height:crypt depth ratio and increased the number and size of the goblet cells relative to villus height. The mucus gel layer coating the surface of the intestinal epithelium is the first barrier to enteric infection against enteric pathogens. Dietary Enzyme Supplementation: 
Dietary enzyme supplementation has become a standard practice in the poultry industry, largely driving by the rising feed ingredient costs, particularly sources of dietary phosphorus, energy, and protein.  Increasing use of grain and oilseed processing co-products that have lower nutrient digestibility has also created greater incentives for use of supplemental enzymes, especially in feeds that are not supplemented with pharmaceutical antimicrobial feed additives. Supplemental enzymes in the feed are used to achieve one or all the following objectives: (a) increase the animal´´´´s own supply (Schaible, 1970); (b) alleviate the adverse effects of antinutritional factors, such as arabinoxylans, b-glucans, etc; (c) render certain nutrients more available for absorption and enhance the energy value of feed ingredients (Classen and Bedford, 1991; Lyons, 1993), and (d) modulate intestinal microflora to a healthier state (Engberg et al., 2004).
The major enzymes used in animal feeds are hydrolytic protease, amylase, lipase, phytase, NSP-degrading enzymes, and cellulase. Commercial enzymes products are typically a blend of several different enzymes that are effective on a wide variety of substrates. The enzymes with proven efficacies for animal husbandry include xylanase, arabinoxylanase, b-glucanase, cellulase, phytase, and mannanase (Ferket, 1992; Choct and Kocher, 2000). Amylase and lipase are enzymes commonly used in corn-SBM based diet to supplement endogenous enzymes of the animal, thus improving nutrient digestibility and growth performance characteristics (Ferket, 1993).  Phytate is a universally antinutrient present in all plant material that irreversibly chelates divalent cations and interferes with amino acid absorption in the gastrointestinal tract of birds. Supplementation of poultry diets with enzyme mixtures, including proteases and amylases, has produced significant improvements in growth performance (Greenwood et al., 2002; Burrows et al., 2002). Greenwood et al. (2002) reported that supplementing a corn-SBM broiler starter diet with an enzyme preparation containing a mixture of xylanase, protease, and amylase increased body weight at 14 and 42 days of age. The effect of exogenous xylanases in improving dietary nutrient availability is more complex than phytase. Endoxylanase degrades the xylan backbone of arabinoxylan into smaller units, which has several beneficial consequences. It renders the xylose units more available to monogastrics (Odetallah, 2000). It disrupts the water holding capacity of the NSP (Scott and Boldaji, 1997), and reduces the viscosity of the digesta in the small intestine (Bedford and Schulze, 1998; Choct et al., 1999). Reduced digesta viscosity increases the diffusion rates of nutrients and endogenous enzymes enabling the bird to digest and absorb more nutrients (Pawlik et al., 1990). Endoxylanase releases entrapped nutrients for the digestion by the endogenous enzymes of the bird (Chesson, 2000). Endoxylanase inhibits the proliferation of the fermentative microorganisms in the small intestine by increasing the digesta passage rate and nutrient digestion (Choct et al., 1999). Thus, nutrient utilization is improved by reducing the competition between the host and its enteric microflora.
Many authors have shown the interaction between pentosans, microflora, and enzyme supplementation. Fischer and Classen (2000) reported that bacterial count from the small intestine of broilers fed wheat-based diets was lower in xylanase-supplemented birds than the unsupplemented ones. Because enzymes supplementation reduces the microbial population in the small intestine (Choct et al., 1995; Dunn, 1996), the entire intestinal ecosystem can change. These conditions in the intestine alter the composition and activity of intestinal microflora (Vukic-Vranjes and Wenk, 1996). When the microflora profile changes after enzyme supplementation, there is a decrease in the adverse effects of microbial fermentation. Some of the adverse effects of active microbial fermentation include: deconjugation of bile salts reducing fat digestion (Langhout, 1999); competition between the host and the microflora for nutrients (Bedford, 1995; Langhout et al., 2000); atrophy of the intestinal villi and enlargement of digestive organs (Brenes et al., 1993; Viveiros et al., 1994). Additionally, Santos (2006) showed that dietary supplementation of NSP-degrading enzymes (endoxylanase and complementary enzymes blends) reduces the adverse effects of dietary NSP on nutrient digestibility, and increases the variety of non-starch oligosaccharides that serve as substrate for a more diverse microflora, thus augmenting the positive effect of NSP on ecosystem stability and discouraging Salmonella colonization in turkeys.
Conclusion
Although AGP have served the poultry industry well in maintaining efficient production and animal welfare, the availability of this valuable production tool will become limited in the future because there will be no such products that will be developed in the future and use of remaining AGP will be constrained because of government regulations or consumer demands. Indeed finding alternatives to AGP to maintain gut health and efficient growth performance in poultry is a priority.  Strategic use of these alternatives is dependent upon understanding their modes of action and how they influence the dynamic enteric ecosystem. A stable enteric ecosystem, particularly in the hind gut of poultry, is essential as symbiotic microflora competitively excludes the adverse effects of more pathogenic species.  Establishment of that stable ecosystem depends on uncompromised early gut development, gut motility conditioning by the structural properties of feed, and strategic use of organic acids, essential oils, prebiotics, probotics, and enzymes. 
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Watch Dr. Ferket´s presentation at the Latin American Poultry Congress in Buenos Aires, Argentina, August 2011: 
 
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