Dear Radka!
You have provided interesting data that are consistent with the results of other researchers. It has long been established that aflatoxin is a toxin in Southeast Asia, where it is warm and humid. However, in European countries and in the east of Russia it is hard to find "Southeast Asia", it is hidden in silos. After harvesting the grass mass, silage ripening begins - fermentation is in progress. This raises the temperature. In case of leakage and penetration of air, the formation of aflatoxin is activated. In some cases, its concentration in corn silage reached 150-270 µg / kg, and in the foci up to 480 µg / kg. Therefore, aflatoxin should not be considered a toxin only in warm countries.
Mycotoxin contamination of raw materials is a routine workflow procedure. But to understand the action of adsorbents of mycotoxins is a science. It is necessary to contrast the tales of sellers of aflatoxins with the demands of science on real evidence.
In vitro methods are not suitable for this. They have a different purpose.
(Manafi et al. 2009, tab. 2).
Table 2
Parts of the mycotoxins bound by commercial adsorbent in vitro, %
Mycotoxins Aflatoxin Ochratoxin T-2 toxin
pH in media
4,5 6,5 4,5 6,5 4,5 6,5
AF 90,7 94,7 - - - -
OA - - 61,7 63,1 - -
T-2 - - - - 74,3 71,3
AF+OA 62,0 65,8 33,7 34,0 - -
AF+T-2 43,2 44,7 - - 46,1 46,4
OA+T-2 - - 34,1 34,1 45,3 47,5
AF+OA+T-2 33,5 32,4 5,3 6,3 9,1 9,7
* AF: B1 aflatoxin - 0,5 mg/kg; OA: Ochratoxin - A 1 mg/kg; T-2: T-2 toxin - 2 mg/kg.
The media of an incubation was presented by a feed suspension in buffer solution