Discussion created on 02/04/2014

Effect of Aflatoxin B1 on the Antioxidant System of Rat Erythrocytes and Corrective Activity of Preparation «Vitakorm-Multysporyn»

Antioxidant system protects cells from damage caused by the action of reactive oxygen (ARO). Excessive formation of ARO in erythrocytes can lead to damage to their structure and violation of biochemical processes. Formation ARO increases under the influence of toxic substances. In extremely toxic compounds include aflatoxins, particularly aflatoxin B1 (AFB1). Investigated the effects of AFB1 on the activity of antioxidant enzymes and the content of TBA-active products in erythrocytes of rats. The study was conducted on white nonlinear male rats, which were divided into 4 groups of 5 animals each. Animals of the first group was used as control. Rats of the second group were injected with AFB1 concentration of 15 mkg/kg daily for 14 days orally. The animals of the third group was administered the same amount of toxin along with the preparation «Vitakorm-Multysporyn». The animals of the fourth group were injected only probiotic preparation. Material for research was animal blood. Blood samples were taken on the 7th and 14th days of the experiment. It was established that the introduction of AFB1 of laboratory animals on the 7th and 14th day of experiment changes the activity of antioxidant enzymes in animal cells. SOD activity decreased by 36 and 41 % on the 7th and 14th day, respectively, compared with control animals. The activity of catalase in erythrocytes of rats in first experimental group decreased by 60 and 55 % on the 7th and 14th day, respectively, compared with the control group. Glutathione peroxidase activity, in contrast, grew under the influence of AFB1. At the 7th day it increased to 29, and on the 14th — 44 %, compared with values in the control group. Glutathione reductase activity on the 7th and 14th day decreased by 63 and 72.5%, respectively, compared with controls. The content of TBA-active products in erythrocytes increased by 31 and 22% on the 7th and 14th day, compared with controls. Also investigated the influence of probiotic preparation «Vitakorm-Multysporyn» on the antioxidant system in rats aflatoxicosis. With a prolonged administration of toxin to rats, to some extent, they has adapted to his action, and the use of the preparation «Vitakorm-Multysporyn» significantly stimulates this adaptation.

Golovchak Kristine
Biochemist
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Zahar Gorbenko Zahar Gorbenko
Research
December 18, 2014
Thanks for a good article. Might I ask how many of the rats died?
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Golovchak Kristine Golovchak Kristine
postgraduate
March 16, 2015
Hello. In this experiment none.
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Zahar Gorbenko Zahar Gorbenko
Research
March 17, 2015
Awesome rats! Because, according to FAO, the single oral AFB1 LD50 for male rats is 5,5-7,2 mg/kg/bw. Was the toxin concentration expressed not in relation to body weight?
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Golovchak Kristine Golovchak Kristine
postgraduate
March 17, 2015
I apologize. It should be micrograms. In the text is a mistake.
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Analía Göttig Analía Göttig
Community Manager
March 25, 2015
Dear members, the original post has already been corrected with the information provided by the author. Regards,
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Golovchak Kristine Golovchak Kristine
postgraduate
March 25, 2015
Thank you for editing, Analia.
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