Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins

Published on: 10/5/2021
Author/s : Ning Xuan 1, Xia Guo 1, Hong-Yan Xie 1, Qi-Nian Lou 2, Xing-Bo Lu 3, Guo-Xia Liu 1 and Jean-Francois Picimbon 1.
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1 Biotechnology Research Center, Shandong Provincial Key Laboratory for Genetic Improvement Cultivation, Ecology and Physiology of Crops, Shandong Academy of Agricultural Sciences, Jinan; 2 Shandong Silkworm Institute, Shandong Academy of Agricultural Sciences, Yantai, and; 3 Plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan, China.

 

We analyzed 20 chemosensory protein (CSP) genes of the silkworm Bombyx mori. We found a high number of retrotransposons inserted in introns. We then analyzed expression of the 20 BmorCSP genes across tissues using quantitative real-time polymerase chain reaction (PCR). Relatively low expression levels of BmorCSPs were found in the gut and fat body tissues. We thus tested the effects of endectocyte insecticide abamectin (B1a and B1b avermectins) on BmorCSP gene expression. Quantitative real-time PCR experiments showed that a single brief exposure to insecticide abamectin increased dramatically CSP expression not only in the antennae but in most tissues, including gut and fat body.

Furthermore, our study showed coordinate expression of CSPs and metabolic cytochrome P450 enzymes in a tissue-dependent manner in response to the insecticide. The function of CSPs remains unknown. Based on our results, we suggest a role in detecting xenobiotics that are then detoxified by cytochrome P450 anti-xenobiotic enzymes.

Key words: Abamectin, Bombyx mori, chemosensory protein, cytochrome P450 oxidase, insecticide, moth.

 

Abstract published in Insect Science (2014) 00, 1–17, DOI 10.1111/1744-7917.12116. This article was awarded as the Most Cited Paper of Insect Science in 2017.

 
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