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Improving protein digestibility in poultry

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Saif Ul Haq Saif Ul Haq
Animal Nutritionist
October 21, 2019

Dear Haroon,
Impressing customers is useless, proteases should perform.
Many factors play, I mean right type & quantity of protease, ample dietary substrate, right physiological age of bird and perhaps most important is the return on investment.
Regards.

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Haroon Mushtaq Haroon Mushtaq
PostDoc in Poultry Nutrition
October 26, 2019

Saif Ul Haq actually, nature of protease should be known to have its effect and per se nature is a wider term so does the performance. We shouldn’t expect consistency of performance from enzymes as there are a lot of factors which deviate performance of enzymes, even phytase, but return on investment matters.

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Alvaro Dubois Alvaro Dubois
Technical Consultant at Cargill
October 24, 2019

One common mistake is to refer to the fact that an enzyme, when added on top of others, will still show benefit (performance, digestibility, etc.) as synergy. Synergy is a special situation that is best represented by the equation 1 + 1 = 3. In other words, when you add the second enzyme you would get more than the sum of their individual effects. In most cases, as in the discussion above on protein digestibility, what can be best expected is to have additivity (1 + 1 = 2) but in most cases you have less than that (1 + 1 = 1.5) or even (1 + 1 = 1). Considering that basically all broiler diets today have an added phytase and a large part also a carbohydrase and that both contribute to an increase in protein digestibility (see the great meta-analysis conducted by Cowieson and Bedford published in 2009 'The effect of phytase and carbohydrase on ileal amino acid digestibility in monogastric diets: complimentary mode of action?'), what can be expected with adding a protease on top of the two is quite likely a less than additive impact since the pool of non-digested proteins was already shortened by phytase and carbohydrase addition.

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Youssef Attia Youssef Attia
Professor
October 25, 2019

Sure the effect of protease will be indeed affected by other enzymes in the enzymes cocktail and type of diet composition due to the space available for protease to work on even with a different mode of action of different enzymes which may affect protein digestibility indirectly.

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Saif Ul Haq Saif Ul Haq
Animal Nutritionist
October 26, 2019

A very informative and practical discussion.

What is the scope of working with proteases while using ingredients of high AA digestibility?

What type of exogenous proteases complement/are synergistic with the endogenous protease?

Do exogenous proteases affect endogenous protease production?

Please comments from Dr. Waldroup.

Regards.

Reply
Mangalmurti Pathak Mangalmurti Pathak
MVSc & A.H. Animal Nutrition
October 29, 2019

I recently conducted a trial using different protease enzymes available in India on commercial male broilers. There was an improvement in performance in all the groups against the control group with 5% reduction in the total protein content and ideal amino acid ratios. In one of the groups where protease enzyme is supplemented with xylanase, the enzyme had better performance than other groups.

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Dimcho Djouvinov Dimcho Djouvinov
Animal Nutritionist
October 30, 2019

If you apply protease as a sole enzyme, you get improvement in performance. When add into diets with phytase and NSPase, there is no additional significant effect. So, 1+1+1 =2.

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Mangalmurti Pathak Mangalmurti Pathak
MVSc & A.H. Animal Nutrition
October 30, 2019

My experience is that in the absence of phytase and xylanase we do not get much improvement if we solely use protease specially in broiler feed.

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Nelson Ruiz Nelson Ruiz
Chemist, MS, PhD
October 30, 2019
Dimcho Djouvinov, your comment is quite interesting, and since you are writing from AB Vista I assume you have quantitative data to support your assertions, would you share your data? Thanks
Reply
Dimcho Djouvinov Dimcho Djouvinov
Animal Nutritionist
November 1, 2019
Nelson Ruiz , thanks for your comment.
My statement is based on meta-analysis performed by Lee et al., 2018: Poultry Sci., 0:1-8. http://dx.doi.org/10.3382/ps/pey042
For details, pls refer to the paper above.

Just an example with AIAAD (%) of Lys:

no other enzymes:
84.21 no protease
86.37 + protease

with other enzymes:
85.75 no protease
86.11 + protease

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Saif Ul Haq Saif Ul Haq
Animal Nutritionist
October 30, 2019
Might be Mr.Djouvinov indicating towards law of diminishing returns in case of application of 3 types of enzyme supplementation.Would like to share his trial details to support his notion.
Regards
Reply
November 14, 2019

That depends on soya quality. If the soya quality is high the improve with xylanase and phytase even with ABvista product not significant but when we have soya with high protein content and less digestible amino acid the improvement will be very nice.

Reply
Naqash Naqash
Mphil Animal Nutrition and Feed science
November 19, 2019

Very informative discussion...
Well as Professor Attia mentioned diet composition also plays a significant role regarding this information, as my knowledge this time we use with cooperation with Chengdu Mytech BiotechCo., LTD manufacture natural safe and stable product.

Reply
Popoola Adesola Popoola Adesola
Student
February 7, 2020
Hello Dr, I will be asking question from different thing entirely.
Please are there correlations among Hen day egg production, egg weight and feed intake in broiler breeder hens?

What happen to egg weight, hen day production and feed intake in aged broiler breeder hens (55-70 weeks).

Do broiler breeders decrease in weight when they reach a particular age.
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