Soft – Plex  Fungi Plex™

The first multiplexing cytometric bead assay kit for quantitative detection of five mycotoxins

FUNGI-PLEX™ is the first, commercially available multiplexed cytometric bead assay kit for simultaneous detection of mycotoxin contamination in food and feed products.

The first release of the kit allows quantitative detection of five mycotoxins simultaneously:

Ochratoxin A

Aflatoxin B1

Fumonisin B1

T2 Toxin

Zearalenone


 Antibodies, specific to the five mycotoxin species are covalently conjugated to six spectrally discrete, *color coded* particles. The coated part ides are used to capture and quantitate the mycotoxin analytes in direct competi­tive immunoassay Samples are acquired in a flow cytometer and the acquired data are automatically analyzed with the FCAP Array software.

Principles:

- Toxins are extracted from a ground sample with 75 ACN. The extracted samples and a coctail of phycoerythirine (PE-conjugated mycotoxines are mixed and added to the antibody-coated micro­spheres. Micotoxins from the extracted sample and PE conjugated mycotoxins compete to bind with the antibody coated to the microbeads. The analyte is then measured by detection of the PE fluorescence-emission with flow cytometric analysis.

- The intensity of the PE fluorescence is directly proportional to the amount of bound conjugates and inversely proportional to the concentration of mycotoxins in the sample. Therefore, as the concentration of mycotoxins in the sample increases, the intensity of the PE fluorescence will decrease. The fluorescence intensities of the samples are compared to the corresponding intensities of the kit standards and the sample concentrations are determined.

How does FUNGI-PLEX™ technology compare with other mycotoxin assay methods?

FUNGI-PLEX™ is the first multiplexing and high throughput method for quantitative detection of mycotoxins.

Speed/High Throughput


 •       FUNGI-PLEX™ addresses the needs of applications requiring both multiplexing and high throughput (up to 1000 samples per day) at the same time.

 •  Oue to robust multiplexing, FUNGI-PLEX delivers more data in less time than the traditional bioassay products, such as the ELISA and Microarray techniques.

Sensitivity. Reliability, Accuracy


* The technology generates accurate quantification of the mycotoxins with high sensitivity
and accuracy at low content rations, dose to the maximum ''''tolerance levels''''of the toxins

•  Because each microsphere serves as an individual test, each measurement represents a large number of bioassays performed and analyzed simultaneously The typital CV is less than 15,

• The FUNGI PLEX™ is highly competitive with the assays detecting only one single toxic compound.

Versatility. Flexibility

•  The matrix effect is low as compared to the ELISA technology, primarily due to the fluorescence based detecting system. Because the matrix effect is low, the FUNGI-PLEX1 technology performs uniformly with a wide range of samples and extraction methods. The kit represents a robust assay relatively tree of interfe­rences for a great variety of agricultural products.

•  The technology can be formatted to regional myco­toxin limits and regulations. The technology details can also be customized for the specific needs of users.

Reproducibility

•  High-volume production FUNGI-PLEX™ microspheres and fluorphore-conjugated mycotoxins within a single lot allows assay standardisation.

•  FUNGI PLEX has a relatively long (>12 months) shelf-life


Simplicity

•  Samples are prepared in a single-step procedure: The extracts are mixed with the PE conjugated detector and with the microbeads in a plate well.

•  No washing step is required: After incubation, the samples are acquired with a flow cytometer

•  Data analysis and report generation Is automatic with FCAP Array™ software. FCAP Array™ software provides special support for high throughput measurement with BDFACSArray™ bioanalyzer

ENGOREPROD 1283-4000 20091121