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Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts?

Published: May 31, 2014
By: Dr. Jaydip Mulik ( M.V.Sc.DCT.MBA)
Introduction
It has been observed that there are lots of controversies in poultry nutrition regarding the use of feed ingredients from Animal source origins. Many poultry experts are against to use animal protein and energy sources such as Meat cum bone meal, Blood Meal, Fish/Fish meal, Animal fat etc in poultry feed.
But now a days due to use of high tech Rendering equipments the byproducts of Abattoirs such as MBM & Animal Fat produced are of best nutritional quality. Also Sterilized fish meal is available with good nutritional values. Those products are sterilized and having a good shelf-life. Due to those properties now its need of poultry feed industry to use those products in poultry feed as a good Protein and energy sources.
Now all poultry feed manufactures are using Meat cum bone meal, Blood Meal, Animal Fat and Fish Meal in poultry feed as a conventional items but some controversies are still there & everybody feel that by use of more Nov- Veg sources in feed may lead to more heath issues related to micro flora such as Salmonella, E-colli, Streptococci, Staphylococci, Clostridia etc .
In concern to this we have conducted the field trials to know the exact bacterial loads carried in feed with use of Non Veg protein sources at its different inclusion levels Viz. 4%, 6% & 8%. 
Materials and Methods
We have selected the different feed ingredients both Veg and Non-Veg to formulate the Broiler Finisher diet which are listed below.
  1. Maize
  2. Groundnut Deoiled Cake
  3. Soya Deoiled Cake
  4. Flaked Full Fat Soya
  5. Meat Cum Bone Meal
  6. Blood Meal
  7. Animal Fat
  8. Rapeseed Deoiled Cake
  9. Maize Gluten meal 
Sampling Method
We are collected the different feed samples for microbiological analysis by “Random Sampling method” and details of which are as.
CONTROL SAMPLES
Batch No.1. Sample Name - Finisher 2 ( Only Veg Sources)
Pure Vegetarian Feed without addition of any Non Veg sources Viz. MBM, Fish Meal(MBM), Blood Meal(BM), Animal Fat(AF) etc.
Batch No. 2. Sample Name - Finisher 2 (4% Non Veg Sources)
4% Non-veg inclusion ( MBM + BM + AF)
Batch No.3. Sample Name - Finisher 2 (8% Non Veg Sources)
8% Non-veg inclusion (MBM + BM + AF)
TEST SAMPLES:
Batch No. 4. Finisher-2(6% Non Veg Source) with addition of Feed Acidifier - I @600 g/ Mts of Feed up to Batch No. 13
Batch No. 14. Sample Name - Finisher 2 (6%Non Veg with addition of Feed Acidifier – I @ 600 g/ Mts)
Batch No. 15. Finisher-2 (6% Non Veg Source) WITHOUT addition of Feed Acidifier - I up to Batch No.. 24
Batch No. 25. Finisher-2 (6% Non Veg Source) with addition of Feed Acidifier - II @500 g/ Mts of Feed up to Batch No. 34
Batch No. 35. Sample Name - Finisher 2 (6%Non Veg with addition of Feed Acidifier – II @ 500g / Mts)
Batch No. 36. Finisher-2 (6% Non Veg Source) with addition of Feed Acidifier - II @1000 g/ Mts of Feed up to Batch No. 40
Batch No. 41. Sample Name - Finisher 2 (6%Non Veg with addition of Feed Acidifier – II @ 1000g / Mts)
Batch No. 42. Finisher-2 (6% Non Veg Source) WITHOUT addition of Feed Acidifier - II up to Batch No.51
Batch No. 52. Finisher-2 (6% Non Veg Source) with addition of Feed Acidifier - III @500 g/ Mts of Feed up to Batch No. 61
Batch No. 62. Sample Name - Finisher 2 (6%Non Veg with addition of Feed Acidifier – III @500g / Mts)
Batch No. 63. Finisher-2 (6% Non Veg Source) with addition of Feed Acidifier - III @1000 g/ Mts of Feed up to Batch No. 67
Batch No. 68. Sample Name - - Finisher 2 (6%Non Veg with addition of Feed Acidifier – III @1000g / Mts) 
Results 
Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts? - Image 1
Discussions
  1. Microbiological analysis shows that feed containing only Veg. Ingredients carries more bacterial count as compare to other samples collected.
  2. There is no any co-relation found as per reports that with increase in inclusion levels of Non-Veg sources in feed, the microbial count increases.
  3. There are deviations in the report of both laboratories as the dates of analysis started are different for same samples.
  4. There is no significant difference in the microbial counts of “Treated and Non Treated Samples”. 
Conclusion
The study clearly indicates that there are some Veg. Feed ingredients in the feed which carries more harmful micro-flora and its need to identify the same.
If Non-Veg protein sources are of good quality then you can use the same utmost in our feed formulations looking after feed economics.
Study found that uses of different feed acidifiers in feed at different doses are not effective to reduce or nullify the microbial count. 
References 
Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts? - Image 2
 
Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts? - Image 3
 
Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts? - Image 4
 
Out of the Box: Which feed ingredient carries more harmful bacteria – veg or non veg? And up to what extent feed acidifiers play a role to reduce those counts? - Image 5
Authors:
Dr Jaydip Mulik
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DrRakesh Purohit
25 de noviembre de 2015
Valuable information
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Tawfik
9 de diciembre de 2014
CAN YOU SEND ME PROGRAMMER I CAN USE IT
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Dr Jaydip Mulik
16 de junio de 2014
Dear All, With reference to above study & to find out the root cause Veg. item/ingredient which carries more harmful micro flora, we have sent all ingredients for microbiological analysis & results of the same are as... Microbiological Analysis Report Sr no. Sample Details Aerobic Plate Count Cfu/gm E.coli Cfu/gm S.aureus Cfu/gm Faecal Streptococci Cfu/gm SRC Cfu/gm Salmonella /25gm 1 Maize 500 NIL NIL NIL NIL Not Detected 2 Maize Gluten Meal 11700 NIL 100 220 NIL Not Detected 3 Soya Deoiled Cake 700 NIL NIL NIL NIL Not Detected 4 Flaked Full Fat Soya 7900 NIL 100 30 NIL Not Detected 5 Rapseed Meal 5300 NIL NIL NIL NIL Not Detected 6 Ground nut Deoiled Cake 1100 NIL 100 NIL NIL Not Detected
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