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Mineral mix for vannamei shrimp

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei

Published: November 8, 2010
By: Eric De Muylder (CreveTec, Belgium)
The white shrimpLitopenaeus vannameihas widely replacedPenaeus monodonas the cultured species. One of the reasons for this change is the fact that L. vannameican be cultured in higher densities and with a lower exchange of water, which increases the bio-security.

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 1

L. vannamei is a euryhaline species and can be cultured in salinities from 0 to 50ppt, although the best growth is obtained in salinities between 10 and 25ppt. In lower salinity culture, there is an osmotic pressure between the shrimp body and the surrounding water, resulting in automatic water uptake mainly through gills and intestine. With lower salinity the shrimp face more difficulties to uptake macro-minerals from the water.

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 2

It has been shown that inclusion of these minerals through the feed can be a solution. However, leaching of these minerals, which are often highly water soluble reduces this option.

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 3

 Therefore, a mix of specific minerals was developed which shows increased retention in the pellets and is available for the shrimp. A growth trial confirms that shrimp show good growth even at low salinities when minerals are added in the feed.

Leaching of minerals from feed pellets

In the first experiment, 12 diets were produced with a different mix of minerals. 98 percent of the ingredients used are the same and two percent minerals were added. Samples of feed were put in water with 0ppt salinity and 25°C for one hour. The minerals in the feed were analysed before and after leaching and corrected for moisture content and overall leaching. A reference diet, (0) without added minerals, was also analysed to have an idea of the retention of minerals from the other ingredients. This gave a retention percentage for each mineral for each sample, which can be seen in Graph 1. It is clear that leaching of minerals is especially important for Na and K, although we can see that some samples show a higher retention than others.

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 4
Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 5
Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 6

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 7

Solubility and digestibility

The lower leaching rate (higher retention) could be the result of a lower solubility of the mineral; which could affect the digestibility of this minerals for the shrimp. Therefore, the apparent digestibility was tested for some selected growth trials. It is clear from Table 1 that for all diets, the digestibility of selected minerals is not the problem.

Material and methods

From the leaching experiment, the most promising combinations were used for a feed trial. Four diets were produced on a small pellet mill with a diameter of two millimeters.

Shrimp (Litopenaeus vannamei) were imported from Thailand and raised to two grams. They were then transferred to the CreveTec-AFT Research station in Venray and divided over 20 nets (volume 150L) in two separate tanks. Shrimp were acclimatised for several weeks to a lower salinity.

Each tank is connected to a biofloc reactor to control water quality. This way, the water quality is equal for all nets. One tank was running on a salinity of 10ppt and the other on 5ppt. Each diet was used in three replicates for the 10ppt salinity and two replicates for the 5ppt salinity.

Each net is equipped with a belt feeder. Feeding was adjusted daily to an estimated growth and percentage of biomass.

Results

During the first two weeks (see table 2) the shrimp were fed continuously through a belt feeder. Growth was good for all replicates. Average growth loss between 10ppt and 5ppt was 4.3 percent.

The most promising diets were diet number two and four. For diet four there was no growth diffference between salinities.

These results show that Litopenaeus vannamei can easily be grown at good growth rates in lower salinities if minerals are added in the diet.

For week three and four the feeding frequency was lowered to only four times per day. This will result in slower feed uptake, longer interaction of feed and water and higher leaching of minerals from the feed. The salinity of tank number two was lowered from five to four ppt.

Growth was affected negatively by the lower feeding frequency and dropped to 1.25g/week (see table 3).

There was no negative influence of lower salinity anymore, maybe because shrimp adapted to this condition. The best growth was obtained with diet number three.

For week five and six the feeding frequency was further lowered to only two times per day.

To understand the influence of leaching on growth, the feed for the three replicates in tank number one was treated before feeding: Four nets received normal feeds; four nets received feeds which were first soaked in fresh water for one hour before feeding and four nets received feeds which were first soaked in tank water (10ppt) for one hour before feeding (see table 4).

There was a clear influence of leaching on growth, indicating that leaching of nutrients is an imported factor in the growth of shrimp. Leaching for one hour in 10ppt water reduces the growth by 15 percent and leaching in fresh water reduces growth by 33 percent.

Week one to six - overall performance (see table 5).

• Growth is negatively affected by lower salinity
• The best growth was observed with diet number two for 10 and lower salinities.

Conclusion

Growth and survival are negatively affected by lower salinity of pond water and by decreasing feeding frequency. The addition of various minerals in feeds makes it possible to still obtain growth in those conditions. Diet number two seems to be the best source of minerals in both 10ppt and lower salinity, which confirms earlier results of leaching and digestibility. Based on these results a special mineral premix was developed jointly by companies Prayon and CreveTec.


This article was originally published in the magazine INTERNATIONAL AQUAFEED (Volume 13 Issue 4 2010) in July-August 2010 by Perendale Publishers (UK). This contribution is greatly appreciated by Engormix.com and its members.

Inclusion of a mineral premix for low-salinity culture of Litopenaeus vannamei - Image 8
Related topics
Authors:
Eric De Muylder
CreveTec
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Jose Kumar
18 de junio de 2021
How much mineral to add 1000000 litre water in ponds .At 10.ppt. salinity.
A. WALTER MOTHA
10 de abril de 2021

Which mineral is the best?

Chetan Patel
14 de marzo de 2019
Which mineral is best for venamei farming?
Kamal Kant
10 de julio de 2018

Which mineral is best for venamei farming?

G S RATH
17 de mayo de 2018
Coating mineral adding a binder to feed is not advisable as this will leachout after some time once put in water. Shrimp hold 2-3 pellets of feed and nibble after completing the same looks for next pellet. My comment in previous discussion is not to add in feed but to add in water periodically weekly once. A good amount of mineral is supplemented through feed formulation.
G S RATH
17 de mayo de 2018
Yes It is very much essential to supplement Min Mix in feed mostly in low saline shrimp culture farming.Minerals should be supplemented on weekly basis.During initial days of culture the dosage will be low compare to peak culture days(>70 Days).In low salinity conditions the availability of minerals are less compare to high saline water. Mineral mixture supplement is more important during moulting period of shrimp,farmer need to have a record of his pond shrimp moulting cycle to get a best result during application of minerals. Hepatopencreas in shrimp plays a prominent role in transport of these minerals during and after moulting cycle in the pond. Before the mouting cycle starts in shrimp the hepatopencreas collects maximum minerals from the pond water and stores with in once the moulting is over it releases back the minerals to body to harden the new soft shell this process takes 8-10 Hr,during this time the weak shrimp reduces the feed intake. So it is essential to supplement mineral regularly depending on the carrying capacity of soil and age of pond. Pls do not interlink white gut to Mineral suppliment both are different. White gut in shrimps are basically due to grigerine parasites or bacterial load in gut. These bacteria and parasites damage the gut lining tissue which appears as white past like and comes out as fecal matters. White gut in shrimp culture ponds are due to high organic load. This need to be drained periodically from pond bottom to control WHITE GUT.
Suresh Varma
9 de marzo de 2017
Low saline water which minerals used in pond water and which minerals feed mixing
prashant naik
13 de septiembre de 2016
Adding minerals in feed, but observed no much growth in low saline water. Wht could be d dose of mineral/kg ?
premnath v
20 de septiembre de 2015

Hi. How can i solve whitecut problems in prawn?

Dr P V S V Prasada Rao
17 de agosto de 2015

I am a consultant scientist, aquaculture with 31 years field experience,did my Ph.d in fisheries in1987,Proprietor of Indo Aqua Technologies feed mill at visakhapatnam ,India.Please call me for any quaries in grow out culture, nutrition, hatchery problems etc.

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