organic acids in animal nutrition

Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)

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By: ADDCON
Title : Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
Date: March 29, 2011
Description: Christian Lückstädt, Technical Manager of Addcon, introduces the book " Standards for acidifiers" at the 1st International Acidifier Summit in Bangkok, Thailand.
Mumbai, Maharashtra, India
Specialist in Animal Nutrition
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/14/2011 | Is there any effect of organic acids on pepsin activity which will ultimately improve protein digestion.
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/14/2011 | Dear Dr. Shyam,
Pepsins are the principle proteases in gastric secretion. The activation of pepsinogen and subsequently of pepsin is acid dependent. Optimal activity of pepsins is at pH 1.8 to 3.5. They are inactivated at a pH of 5. Since the proteases are responsible for the digestion of proteins it has been shown that acidification with organic acids can improve protein digestibility, for instance from 86% up to 90% (apparent digestibilty improvement after formic acid use).
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Chennai, Tamil Nadu [Madras] , India
Business Development Manager
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/28/2011 | Dear Dr.Luckstadt

Can you pls elaborate the mode of action organic acid in shrimp digestion.

Regards
T.Gnanamani
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/28/2011 | Dear Mr. Gnanamani,
we have seen promising effects in shrimp (L. vannamei) with the use of dietary organic acid salts, in particular potassium diformate, especially on weight gain, survival rate and FCR - thus productivity is significantly enhanced, as demonstrated last week at the Asian Fisheries and Aquaculture Forum in Shanghai. The reduction of mortality is clearly a results of the inhibition of pathogenic bacteria, like Vibrio. The improvement of FCR might be explained by improved digestibility. I may speculate here on impacts on the hepato-pancreas after altering the pH in the neutral GI-tract of the shrimp.
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Santiago, Region Metropolitana, Chile
Consultant
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/28/2011 | Dear Dr. Luckstadt:
are this organic acids recommended on pet foods? which are mainly recommended and what are the effects on palatability?
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
04/28/2011 | Dear Mr. Arancibia,
no, formic acid or formate based additives are not recommended for use in pet food.
They can be safely used in swine, poultry, fish, shrimp and calve.
In swine a spanish study recently proved that potassium diformate is increasing palatability of diets. There were significant effects on improved feed preference
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Amman, `Amman, Jordan
Marketing Representative
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
08/30/2011 | what is the mode of action for formic acid in damging of salmonella ?
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
08/31/2011 |

Organic acids, like formic acids, have to ways in inhibiting the growth of gram-negative bacteria, like Salmonella, E.coli, Campylobacter etc. One is via a pH-decrease of the environment in which the bacteria strives. Bacteria have a certain pH-optima in which they can grow. For Salmonella the minimum pH in which it can still grow may be around 4.5; but the optimum is certainly nearer to neutral. So even if you don't reach pH-levels below 4.5 you are able to increase the generation time (in other words make the Salmonella less quick in reproducing) of the bacteria. The second way of inhibiting the bacteria is by penetrating its cell membrane (works only fro gram-negative bacteria). The undisscociated part of the acid is able to penetrate this membrane and can release H+ ions into cytoplasm of the cell. This will lead to a counter-reaction of the cell (pumping H+ ions out with the use of energy. This energy expenditure will weaken the bacteria and may lead ultimatively to cell death. Formic acid in particulra has shown in several MIC-studies that it is among the strongest acids to stop growth of for instance Salmonella!

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Ho Chi Minh, Vietnam
Agro chemist
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
09/20/2011 | Dear Dr. Luckstadt,
I am concerning about quality control in the lab: what is the analytical method to analyse diformate content in feed sample?

best regards,
Hoa Tran
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
09/20/2011 | Dear Mr. Tran,
you can eitehr use titration methods (ISO 2114) to measure the amount of formic acid - and based on that calculate the amount of diformate content (from the CoA of a diformate you would know the amount of formic acid in a given diformate product). This method can be carried out by nearly all labs world-wide. If you need to measure diformate in a matrix, like compound feed, you would need to do an ion chromatography analysis.
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Marilao, Bulacan, Philippines
Specialist in Animal Nutrition
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Dr. Luckstadt.

I appreciated the info you brought about regarding diformate and how it can be measured. Is it also possible for other formic salts like calcium formate? How does the two compares both for activies and effectivity especially for swine? How would we know the adequete dosage or volume to be used for both organic acids? Thanks in advance. I had been using organic acids as per manufacturer's prescription but am i using it right or am i maximizing the suppose benefit of such products?

Respectfully,
Rodel Villaraza
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Abuja Capital Territory, Nigeria
Animal feed producer
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | What are the significant effect in aqua feeds especially in extruded ? can extrusion process affect its performance and the recommended inclusion levels
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Kathmandu, Bagmati, Nepal
Marketing Manager
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Dr.Christian,
I really appreciate your prompt reply in this forum on organic acids. Since pH of GIT of poultry at foregut & hindgut varies considerably I doubt a single organic acid will give the desired level of pH 4.5-5 through out the GIT of poultry.
In our experience product containing right combination of organic acids having different pKa value has showed good activities to inhibit bacterial growth as well as helped in digestion of feed.
Would you recommend single organic acid or combination of selected organic acids as an acidifier for poultry?
Thanks !

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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Mr. Villaraza,
diformates - in your case potassium diformate are double salts, thus the structure is a combined molecule of formic acid and formate, whereas Ca-formate is only a formate. You therefore have no pH-decreasing effect when using Ca-formate only, which is especially important in weaned piglets, since their stomach is still inadequately producing acid in the stomach - you are therefore in need for a molecule which can decrease the pH. We have been recently running trials, also in the Philippines in order to compare potassium diformate and Ca-formate. The effect supported an already earlier published study, which prove that potassium diformate is increasing pig performance on average of 59 trials by 3.52% (feed intake), 8.67% (weight gain) and 4.2% (FCR), while Ca-formate is decreasing feed intake on average of 26 trials by 0.59%, improving weight gain by 1.68% and improving FCR by 1.39%. The study (holo-analysis) looked at all available published studies with acidifier in pigs - so it gives a very good overview on the efficacy of organic acids in swine.
An adequate dosage is always tricky to suggest, but i would suggest to use not less than 4 kg/t in order to have a significant impact. Should you have further questions you may contact our consultant in the Philippines under karsten.schroeder@addcon.com. He is located in Cebu.
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Mr. Saidat,
orgamic acids per se are volatile and thus would have high losses during pelleting or extrusion process. Organic acids salts on the other hand are more stable. We have been testing our organic acid doulbe salt for the aquaculture sector, potassium diformate, at the Aquaculture Protein Center in Norway under high extrusion temperatures (above 120°C). The effect reveal that the digestibility of several essential amino acids was improved after using potassium diformate. Thus we can clearly see significant effects of the additive in extruded diet - it also shows that the additive is surviving the extrusion process. The study has been published at the ISFNF (Fish Nutrition and Feeding symposium) 2010 from Storebakken et al.
In aquaculture diets, especially in the tropical aquaculture we recommend to use between 2-5 kg/t, depending on species and bacterial conditions in the farm/pond.
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Mangalore, Karnataka, India
Animal Nutritionist
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Hello Dr. Luckstadt

Thanks for the beautiful info that you are providing on the use of organic acids in animal feeds. Recently, we have conducted trails on the use of lactic acid for growth and health monitoring in rohu fish (L.rohita). We have tried at 1%, 2% and 3% incorporation levels. 3% level was found be best in giving highest specific growth rate and super oxide anion production as indicated by highest NBT value (Nitroblue tetrazolium). The gut histology revealed mucosal layer being thrown in to multi-folds increasing absorptive area in the fish fed with 3%LA. Also, when challenged to Aeromonas hydrophila the 3% LA administered fishes showed highest relative survival percentage indicating their efficacy in enhancing their growth and immunity. This study was possible because of providing the relevant literature from your side. We sincerely acknowledge the help rendered by you.
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Mr. Dahal,
the desired effect of organic acids in the intestine of poutlry is not to acidify! You cannot and shall not reach pH levels of 4.5-5 in the duodenum, jejunum, caecum etc. This would also impair teh effect of digestive enzymes over there. Thus, the inhibition of pathogenic bacteria in the intestine does not come from an acidifying effect of the intestine, but from a direct anti-microbial effect of the organic acid. The undissosciated form of the acid, as been reported before, is able to pass through the cell membrane of gram negative bacteria (Salmonella, Campylobacter, E.coli for instance) and start a cascade of effects inside the bacteria cell, which will ultimately lead to the cell death. Acids with different pKa levels will have different levels of disscociated and undissociated forms present. However in my mind it is more imporant to show that the chemical molecule is acutally reaching the intestine, which a lot of acids has been failed to do so. Diformates on the other could demonstrate that 85% of its active ingredient is reaching the duodenum, thus having a strong anti-bacterial effect over there. This has been confirmed by an upcoming DEFRA study, which tested the effect of diformate for instance in caecal content.
So in general - the use of organic acids in poultry is certainly a good decision. You have to make sure that you are using a product with a high load of active ingredients, which are also reaching the desired places of action.
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/08/2011 | Dear Dr. Ettiqi,
thank you for the nice comments on using lactic acid in rohu. As I am always interested to see new results on the use of acidifier in fish, which I think is one of the upcoming additives in current aquaculture, would you mind sending me some data, if published?
You may contact me under christian.lueckstaedt@addcon.com
Thanks!
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Kathmandu, Bagmati, Nepal
Marketing Manager
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/09/2011 | Dear Dr.Christian,
Thanks once again !
There is no iota of doubt that lowering pH & ability to inter into cell membrane to release H+ ion into cytoplasm of gram negative bacteria should be the key trait for selecting organic acids to prepare acidifiers.
Shall we select those organic acids/salts in combination which are much capable of disassociating within the bacterial cytoplasm, increasing the cellular hydrogen ion concentration so that product could be able to work in foregut as well as hindgut?
Please also inform us required dose of Diformate to achieve 85% activities at hindgut of broilers, layers & breeders.
Your response is solicited.
Regards,
G.K.Dahal
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Bonn, Nordrhein-Westfalen, Germany
Agricultural Engineer
Porsgrunn, Germany
Re: Forum: Use of Organic Acids in animal nutrition. C. Luckstadt (Addcon)
11/09/2011 | Dear Mr. Dahal,
selecting acids based on pKa-value only (it describes the level amount of acids which is dissociated/undissociated at a certain pH) is not always helpful. In my opinion it is more important that the acids reaching the duodenum/jejunum and caecum - thus the place where most of the nutrients will be digested (and thus also the place where most pathogenic bacteria may strive). With diformates this has been demonstrated. A dosage of 0.3% has been proven to be very effective. However, local dosage recommandation may vary, since in practice often combinations of additives are used. It would be a bit too far stretched to go into detail here. I would therefore like to recommend to you to get in touch with my colleague Dr. Anant Deshpande from India, who is handling the Nepalese marekt for us. You may contact him under: anant.deshpande@addcon.com
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