OPTIMIZATION OF PROCEDURE TO DETECT FOOT AND MOUTH DISEASE VIRUS IN BOVINE ANIMALS OF BALOCHISTAN

Published: November 18, 2011

Source : Asadullah, Jamil Ahmad, Ferhat Abbas and Muhammad Azam Kakar

Summary

Foot and Mouth Disease Virus causes great economic losses in terms of lowered productivity, weight loss, decreased production and mortality in young animals due to myocarditis. Even after vaccination we are not getting desirable results and there are number of outbreaks of the disease. This is because, FMD virus has seven serotypes O, A, C, SAT 1, SAT 2, SAT 3 and Asia 1. All these types have further sub types/ topotypes. Vaccination against one serotype does not provide protection against other serotype, that’s why disease outbreaks occur even after vaccination. An animal once infected with one serotype is equally susceptible to other serotype of the virus. Typical cases of FMD are characterized by a vesicular condition of feet, buccal mucosa and in females, the mammary glands. Clinical signs can vary from mild to severe, and fatalities may occur especially in young animals. FMD viruses may occur in all the secretions and excretions of acutely infected animals. Including expired air. Transmission is generally effected by direct contact between infected and susceptible animals or, more rarely, indirect exposure of susceptible animals to the excretions and secretions of acutely infected animals or uncooked meat products. Mastitis is a common sequel of FMD in dairy cattle. Therefore, it is the matter of urgency to screen out the serotype of FMDV present in Balochistan which has dense cattle population. So that vaccination should be done with specific serotype present in the particular area. This will help controlling the disease and economic losses occurring due to FMD in Balochistan. Epithelial tissue and vesicular fluid samples from mouth lesions of diseased animals were collected from the cattle population Balochistan. RNA of Foot and mouth Disease Virus was extracted from the epithelial tissue and vesicular fluid collected from the diseased animals during disease outbreaks. Standard procedures for the RNA extraction with little modification according to the requirement were adopted later cDNA were synthesized from RNA extracted from FMDV samples using standard procedures described by as per manufacturer’s instructions of the kit used for cDNA synthesis. Keywords: FMDV, bovine, detection, Balochistan

Content from the event: