Dear Jwander Luka:
Serotyping of MDV can be done by immunofluorescence or immunohistochemistry if you have the virus in cell culture using monoclonal antibodies. If DNA is available PCR can be used with serotype-specific primers. ELISA is used for serology to detect antibodies, but is in general not serotype specific.
Matrenal antibodies will be present against serotype one by exposure to field strains or serotype 1 vaccines (e.g., Rispens vaccine), serotype 2 by field exposure or vaccination
(e.g., SB-1) and if vaccinated with HVT to seroptype 3.
Maternal antibodies have little effect on vaccination
as long as cell-associated vaccines are used. If HVT maternal antibodies are present it will affect vaccination
with cell-free HVT.